Experiment: 36hr Phyto Lib Only
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS05575 and BPHYT_RS05580 are separated by 103 nucleotides BPHYT_RS05580 and BPHYT_RS05585 are separated by 181 nucleotides BPHYT_RS05585 and BPHYT_RS05590 are separated by 2 nucleotides BPHYT_RS05590 and BPHYT_RS05595 are separated by 155 nucleotides
BPHYT_RS05575: BPHYT_RS05575 - Fis family transcriptional regulator, at 1,279,406 to 1,280,806
_RS05575
BPHYT_RS05580: BPHYT_RS05580 - hypothetical protein, at 1,280,910 to 1,281,179
_RS05580
BPHYT_RS05585: BPHYT_RS05585 - hypothetical protein, at 1,281,361 to 1,281,759
_RS05585
BPHYT_RS05590: BPHYT_RS05590 - trehalose-6-phosphate synthase, at 1,281,762 to 1,283,198
_RS05590
BPHYT_RS05595: BPHYT_RS05595 - multidrug MFS transporter, at 1,283,354 to 1,284,727
_RS05595
Position (kb) 1281
1282
1283
1284 Strain fitness (log2 ratio) -1
0
1
2 at 1280.829 kb on + strand at 1280.829 kb on + strand at 1280.830 kb on - strand at 1280.903 kb on + strand at 1280.909 kb on + strand at 1280.909 kb on + strand at 1280.910 kb on - strand at 1280.920 kb on - strand at 1280.920 kb on - strand at 1280.920 kb on - strand at 1280.920 kb on - strand at 1280.920 kb on - strand at 1281.030 kb on - strand, within BPHYT_RS05580 at 1281.030 kb on - strand, within BPHYT_RS05580 at 1281.170 kb on - strand at 1281.170 kb on - strand at 1281.231 kb on - strand at 1281.237 kb on + strand at 1281.238 kb on - strand at 1281.238 kb on - strand at 1281.269 kb on - strand at 1281.269 kb on - strand at 1281.269 kb on - strand at 1281.290 kb on + strand at 1281.291 kb on - strand at 1281.515 kb on - strand, within BPHYT_RS05585 at 1281.722 kb on - strand at 1281.722 kb on - strand at 1281.925 kb on + strand, within BPHYT_RS05590 at 1281.949 kb on - strand, within BPHYT_RS05590 at 1281.979 kb on - strand, within BPHYT_RS05590 at 1281.987 kb on + strand, within BPHYT_RS05590 at 1281.987 kb on + strand at 1281.987 kb on + strand, within BPHYT_RS05590 at 1281.988 kb on - strand, within BPHYT_RS05590 at 1281.991 kb on - strand, within BPHYT_RS05590 at 1281.991 kb on - strand, within BPHYT_RS05590 at 1282.077 kb on + strand, within BPHYT_RS05590 at 1282.156 kb on - strand, within BPHYT_RS05590 at 1282.299 kb on + strand, within BPHYT_RS05590 at 1282.299 kb on + strand, within BPHYT_RS05590 at 1282.300 kb on - strand, within BPHYT_RS05590 at 1282.404 kb on + strand, within BPHYT_RS05590 at 1282.404 kb on + strand, within BPHYT_RS05590 at 1282.405 kb on - strand, within BPHYT_RS05590 at 1282.406 kb on + strand, within BPHYT_RS05590 at 1282.407 kb on - strand, within BPHYT_RS05590 at 1282.447 kb on - strand, within BPHYT_RS05590 at 1282.447 kb on - strand, within BPHYT_RS05590 at 1282.875 kb on + strand, within BPHYT_RS05590 at 1283.099 kb on + strand at 1283.100 kb on - strand at 1283.437 kb on - strand at 1283.831 kb on + strand, within BPHYT_RS05595 at 1284.126 kb on + strand, within BPHYT_RS05595
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr Phyto Lib Only remove 1,280,829 + -0.2 1,280,829 + +0.0 1,280,830 - -0.3 1,280,903 + -0.7 1,280,909 + -0.3 1,280,909 + +1.1 1,280,910 - +0.3 1,280,920 - +0.4 1,280,920 - +0.6 1,280,920 - +0.4 1,280,920 - +0.6 1,280,920 - +0.5 1,281,030 - BPHYT_RS05580 0.44 -0.5 1,281,030 - BPHYT_RS05580 0.44 +0.3 1,281,170 - +1.2 1,281,170 - +1.5 1,281,231 - +1.0 1,281,237 + +0.6 1,281,238 - +1.4 1,281,238 - +0.8 1,281,269 - +0.9 1,281,269 - +1.7 1,281,269 - +1.6 1,281,290 + +0.6 1,281,291 - +1.4 1,281,515 - BPHYT_RS05585 0.39 +0.5 1,281,722 - +1.7 1,281,722 - +1.3 1,281,925 + BPHYT_RS05590 0.11 +1.5 1,281,949 - BPHYT_RS05590 0.13 +1.3 1,281,979 - BPHYT_RS05590 0.15 +0.4 1,281,987 + BPHYT_RS05590 0.16 +1.5 1,281,987 + +1.4 1,281,987 + BPHYT_RS05590 0.16 +1.9 1,281,988 - BPHYT_RS05590 0.16 +1.9 1,281,991 - BPHYT_RS05590 0.16 +1.3 1,281,991 - BPHYT_RS05590 0.16 +1.5 1,282,077 + BPHYT_RS05590 0.22 +1.3 1,282,156 - BPHYT_RS05590 0.27 +2.2 1,282,299 + BPHYT_RS05590 0.37 +1.1 1,282,299 + BPHYT_RS05590 0.37 +1.7 1,282,300 - BPHYT_RS05590 0.37 +1.4 1,282,404 + BPHYT_RS05590 0.45 +1.6 1,282,404 + BPHYT_RS05590 0.45 +1.5 1,282,405 - BPHYT_RS05590 0.45 +1.1 1,282,406 + BPHYT_RS05590 0.45 +1.4 1,282,407 - BPHYT_RS05590 0.45 +1.8 1,282,447 - BPHYT_RS05590 0.48 +1.4 1,282,447 - BPHYT_RS05590 0.48 +1.5 1,282,875 + BPHYT_RS05590 0.77 +1.4 1,283,099 + +1.7 1,283,100 - +1.4 1,283,437 - -1.3 1,283,831 + BPHYT_RS05595 0.35 +1.3 1,284,126 + BPHYT_RS05595 0.56 -0.1
Or see this region's nucleotide sequence