Experiment: 36hr Phyto Lib Only
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS05545 and BPHYT_RS05550 are separated by 99 nucleotides BPHYT_RS05550 and BPHYT_RS05555 overlap by 4 nucleotides BPHYT_RS05555 and BPHYT_RS05560 are separated by 362 nucleotides
BPHYT_RS05545: BPHYT_RS05545 - nitrate ABC transporter ATP-binding protein, at 1,274,077 to 1,275,423
_RS05545
BPHYT_RS05550: BPHYT_RS05550 - hypothetical protein, at 1,275,523 to 1,276,092
_RS05550
BPHYT_RS05555: BPHYT_RS05555 - hypothetical protein, at 1,276,089 to 1,276,667
_RS05555
BPHYT_RS05560: BPHYT_RS05560 - Fis family transcriptional regulator, at 1,277,030 to 1,278,088
_RS05560
Position (kb) 1275
1276
1277 Strain fitness (log2 ratio) -2
-1
0
1
2 at 1275.376 kb on + strand at 1275.833 kb on + strand, within BPHYT_RS05550 at 1275.833 kb on + strand, within BPHYT_RS05550 at 1276.386 kb on - strand, within BPHYT_RS05555 at 1276.750 kb on - strand at 1276.750 kb on - strand at 1276.781 kb on + strand at 1276.781 kb on + strand at 1276.781 kb on + strand at 1276.788 kb on - strand at 1276.937 kb on - strand at 1276.969 kb on - strand at 1277.085 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr Phyto Lib Only remove 1,275,376 + -1.8 1,275,833 + BPHYT_RS05550 0.54 -0.9 1,275,833 + BPHYT_RS05550 0.54 -1.0 1,276,386 - BPHYT_RS05555 0.51 +1.2 1,276,750 - +1.1 1,276,750 - +1.1 1,276,781 + +0.0 1,276,781 + -1.4 1,276,781 + +0.3 1,276,788 - -0.2 1,276,937 - -0.7 1,276,969 - -0.6 1,277,085 + +1.8
Or see this region's nucleotide sequence