Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS30505

Experiment: 24hr Phyto Lib Only

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS30495 and BPHYT_RS30500 overlap by 4 nucleotidesBPHYT_RS30500 and BPHYT_RS30505 overlap by 4 nucleotidesBPHYT_RS30505 and BPHYT_RS30510 are separated by 24 nucleotides BPHYT_RS30495: BPHYT_RS30495 - oxidoreductase, at 2,400,935 to 2,402,272 _RS30495 BPHYT_RS30500: BPHYT_RS30500 - ABC transporter permease, at 2,402,269 to 2,403,177 _RS30500 BPHYT_RS30505: BPHYT_RS30505 - ABC transporter permease, at 2,403,174 to 2,403,968 _RS30505 BPHYT_RS30510: BPHYT_RS30510 - aldehyde dehydrogenase, at 2,403,993 to 2,405,420 _RS30510 Position (kb) 2403 2404Strain fitness (log2 ratio) -1 0 1 2at 2402.263 kb on - strandat 2402.453 kb on - strand, within BPHYT_RS30500at 2402.453 kb on - strand, within BPHYT_RS30500at 2402.660 kb on + strand, within BPHYT_RS30500at 2402.660 kb on + strand, within BPHYT_RS30500at 2402.674 kb on + strand, within BPHYT_RS30500at 2402.800 kb on - strand, within BPHYT_RS30500at 2402.800 kb on - strand, within BPHYT_RS30500at 2403.062 kb on - strand, within BPHYT_RS30500at 2403.253 kb on + strandat 2403.559 kb on - strand, within BPHYT_RS30505at 2403.991 kb on - strandat 2404.546 kb on + strand, within BPHYT_RS30510

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Per-strain Table

Position Strand Gene LocusTag Fraction 24hr Phyto Lib Only
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2,402,263 - -0.1
2,402,453 - BPHYT_RS30500 0.20 -0.9
2,402,453 - BPHYT_RS30500 0.20 -0.5
2,402,660 + BPHYT_RS30500 0.43 +1.0
2,402,660 + BPHYT_RS30500 0.43 -0.1
2,402,674 + BPHYT_RS30500 0.45 -1.4
2,402,800 - BPHYT_RS30500 0.58 -0.2
2,402,800 - BPHYT_RS30500 0.58 +0.7
2,403,062 - BPHYT_RS30500 0.87 +1.8
2,403,253 + +1.0
2,403,559 - BPHYT_RS30505 0.48 -1.4
2,403,991 - +1.4
2,404,546 + BPHYT_RS30510 0.39 -0.0

Or see this region's nucleotide sequence