Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS30400

Experiment: 24hr Phyto Lib Only

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS30395 and BPHYT_RS30400 are separated by 39 nucleotidesBPHYT_RS30400 and BPHYT_RS30405 are separated by 238 nucleotides BPHYT_RS30395: BPHYT_RS30395 - small mechanosensitive ion channel protein MscS, at 2,374,231 to 2,375,700 _RS30395 BPHYT_RS30400: BPHYT_RS30400 - amidohydrolase, at 2,375,740 to 2,377,608 _RS30400 BPHYT_RS30405: BPHYT_RS30405 - hydrolase, at 2,377,847 to 2,378,482 _RS30405 Position (kb) 2375 2376 2377 2378Strain fitness (log2 ratio) -1 0 1 2at 2374.868 kb on + strand, within BPHYT_RS30395at 2375.620 kb on - strandat 2375.672 kb on + strandat 2375.758 kb on - strandat 2375.984 kb on + strand, within BPHYT_RS30400at 2376.066 kb on + strand, within BPHYT_RS30400at 2376.554 kb on + strand, within BPHYT_RS30400at 2376.807 kb on - strand, within BPHYT_RS30400at 2376.873 kb on - strand, within BPHYT_RS30400at 2377.653 kb on - strandat 2377.948 kb on - strand, within BPHYT_RS30405at 2378.536 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 24hr Phyto Lib Only
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2,374,868 + BPHYT_RS30395 0.43 +1.8
2,375,620 - -1.7
2,375,672 + -0.1
2,375,758 - +0.2
2,375,984 + BPHYT_RS30400 0.13 -0.9
2,376,066 + BPHYT_RS30400 0.17 -0.6
2,376,554 + BPHYT_RS30400 0.44 -0.8
2,376,807 - BPHYT_RS30400 0.57 +0.8
2,376,873 - BPHYT_RS30400 0.61 -1.7
2,377,653 - +0.4
2,377,948 - BPHYT_RS30405 0.16 +1.4
2,378,536 + +0.2

Or see this region's nucleotide sequence