Experiment: 24hr Phyto Lib Only
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS28875 and BPHYT_RS28880 overlap by 4 nucleotides BPHYT_RS28880 and BPHYT_RS28885 are separated by 24 nucleotides BPHYT_RS28885 and BPHYT_RS28890 are separated by 73 nucleotides BPHYT_RS28890 and BPHYT_RS28895 are separated by 21 nucleotides
BPHYT_RS28875: BPHYT_RS28875 - 5-oxo-1,2,5-tricarboxylic-3-penten acid decarboxylase, at 2,027,966 to 2,028,739
_RS28875
BPHYT_RS28880: BPHYT_RS28880 - 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase, at 2,028,736 to 2,029,494
_RS28880
BPHYT_RS28885: BPHYT_RS28885 - betaine-aldehyde dehydrogenase, at 2,029,519 to 2,030,976
_RS28885
BPHYT_RS28890: BPHYT_RS28890 - 3,4-dihydroxyphenylacetate 2,3-dioxygenase, at 2,031,050 to 2,031,913
_RS28890
BPHYT_RS28895: BPHYT_RS28895 - 5-carboxymethyl-2-hydroxymuconate isomerase, at 2,031,935 to 2,032,336
_RS28895
Position (kb) 2029
2030
2031 Strain fitness (log2 ratio) -2
-1
0
1 at 2028.782 kb on - strand at 2029.022 kb on - strand, within BPHYT_RS28880 at 2029.102 kb on + strand, within BPHYT_RS28880 at 2029.148 kb on - strand, within BPHYT_RS28880 at 2029.879 kb on - strand, within BPHYT_RS28885 at 2031.449 kb on - strand, within BPHYT_RS28890 at 2031.744 kb on - strand, within BPHYT_RS28890
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr Phyto Lib Only remove 2,028,782 - +0.4 2,029,022 - BPHYT_RS28880 0.38 -1.3 2,029,102 + BPHYT_RS28880 0.48 -0.8 2,029,148 - BPHYT_RS28880 0.54 -1.9 2,029,879 - BPHYT_RS28885 0.25 -1.5 2,031,449 - BPHYT_RS28890 0.46 -0.5 2,031,744 - BPHYT_RS28890 0.80 +0.1
Or see this region's nucleotide sequence