Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS25045

Experiment: 24hr Phyto Lib Only

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS25035 and BPHYT_RS25040 are separated by 197 nucleotidesBPHYT_RS25040 and BPHYT_RS25045 are separated by 133 nucleotidesBPHYT_RS25045 and BPHYT_RS25050 are separated by 113 nucleotides BPHYT_RS25035: BPHYT_RS25035 - long-chain fatty acid--CoA ligase, at 1,186,556 to 1,188,253 _RS25035 BPHYT_RS25040: BPHYT_RS25040 - hypothetical protein, at 1,188,451 to 1,188,741 _RS25040 BPHYT_RS25045: BPHYT_RS25045 - LysR family transcriptional regulator, at 1,188,875 to 1,189,786 _RS25045 BPHYT_RS25050: BPHYT_RS25050 - 3-isopropylmalate dehydrogenase, at 1,189,900 to 1,191,030 _RS25050 Position (kb) 1188 1189 1190Strain fitness (log2 ratio) -1 0 1at 1188.330 kb on + strandat 1188.331 kb on - strandat 1188.960 kb on + strandat 1189.024 kb on - strand, within BPHYT_RS25045at 1189.024 kb on - strand, within BPHYT_RS25045at 1189.585 kb on - strand, within BPHYT_RS25045at 1189.585 kb on - strand, within BPHYT_RS25045at 1189.796 kb on + strandat 1189.831 kb on - strandat 1189.860 kb on + strandat 1189.861 kb on - strandat 1189.909 kb on + strandat 1190.312 kb on - strand, within BPHYT_RS25050

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Per-strain Table

Position Strand Gene LocusTag Fraction 24hr Phyto Lib Only
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1,188,330 + +0.1
1,188,331 - -0.3
1,188,960 + -0.3
1,189,024 - BPHYT_RS25045 0.16 -1.0
1,189,024 - BPHYT_RS25045 0.16 -0.3
1,189,585 - BPHYT_RS25045 0.78 -1.3
1,189,585 - BPHYT_RS25045 0.78 -0.1
1,189,796 + -0.9
1,189,831 - +0.4
1,189,860 + +1.3
1,189,861 - +0.5
1,189,909 + -0.7
1,190,312 - BPHYT_RS25050 0.36 +0.1

Or see this region's nucleotide sequence