Experiment: 24hr Phyto Lib Only
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS10790 and BPHYT_RS10795 are separated by 46 nucleotides BPHYT_RS10795 and BPHYT_RS10800 are separated by 176 nucleotides BPHYT_RS10800 and BPHYT_RS10805 overlap by 4 nucleotides
BPHYT_RS10790: BPHYT_RS10790 - C4-dicarboxylate ABC transporter substrate-binding protein, at 2,452,334 to 2,453,686
_RS10790
BPHYT_RS10795: BPHYT_RS10795 - ProQ activator of osmoprotectant transporter ProP, at 2,453,733 to 2,454,488
_RS10795
BPHYT_RS10800: BPHYT_RS10800 - LysR family transcriptional regulator, at 2,454,665 to 2,455,588
_RS10800
BPHYT_RS10805: BPHYT_RS10805 - MFS transporter, at 2,455,585 to 2,456,961
_RS10805
Position (kb)
2454
2455
2456 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2453.704 kb on - strand at 2454.521 kb on - strand at 2454.581 kb on + strand at 2454.743 kb on - strand at 2454.857 kb on + strand at 2454.866 kb on + strand, within BPHYT_RS10800 at 2454.866 kb on + strand, within BPHYT_RS10800 at 2454.867 kb on - strand, within BPHYT_RS10800 at 2454.867 kb on - strand, within BPHYT_RS10800 at 2455.183 kb on - strand, within BPHYT_RS10800 at 2455.586 kb on + strand at 2455.899 kb on + strand, within BPHYT_RS10805 at 2455.899 kb on + strand, within BPHYT_RS10805 at 2456.130 kb on - strand, within BPHYT_RS10805 at 2456.423 kb on + strand, within BPHYT_RS10805 at 2456.496 kb on - strand, within BPHYT_RS10805
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr Phyto Lib Only remove 2,453,704 - +0.1 2,454,521 - -1.0 2,454,581 + -1.1 2,454,743 - -1.1 2,454,857 + +1.8 2,454,866 + BPHYT_RS10800 0.22 +0.6 2,454,866 + BPHYT_RS10800 0.22 +0.3 2,454,867 - BPHYT_RS10800 0.22 -0.1 2,454,867 - BPHYT_RS10800 0.22 -0.0 2,455,183 - BPHYT_RS10800 0.56 -0.6 2,455,586 + -0.2 2,455,899 + BPHYT_RS10805 0.23 -2.6 2,455,899 + BPHYT_RS10805 0.23 -0.2 2,456,130 - BPHYT_RS10805 0.40 -0.1 2,456,423 + BPHYT_RS10805 0.61 -0.3 2,456,496 - BPHYT_RS10805 0.66 -0.2
Or see this region's nucleotide sequence