Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS34095

Experiment: 24hr Phyto Lib Only

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS34090 and BPHYT_RS34095 are separated by 119 nucleotidesBPHYT_RS34095 and BPHYT_RS34100 are separated by 108 nucleotides BPHYT_RS34090: BPHYT_RS34090 - polyamine ABC transporter substrate-binding protein, at 3,245,820 to 3,247,097 _RS34090 BPHYT_RS34095: BPHYT_RS34095 - spermidine/putrescine ABC transporter substrate-binding protein, at 3,247,217 to 3,248,266 _RS34095 BPHYT_RS34100: BPHYT_RS34100 - spermidine/putrescine ABC transporter ATP-binding protein, at 3,248,375 to 3,249,472 _RS34100 Position (kb) 3247 3248 3249Strain fitness (log2 ratio) -1 0 1at 3246.487 kb on + strand, within BPHYT_RS34090at 3246.488 kb on - strand, within BPHYT_RS34090at 3246.885 kb on - strand, within BPHYT_RS34090at 3247.168 kb on - strandat 3247.401 kb on + strand, within BPHYT_RS34095at 3247.440 kb on + strand, within BPHYT_RS34095at 3247.501 kb on - strand, within BPHYT_RS34095at 3247.570 kb on - strand, within BPHYT_RS34095at 3247.762 kb on - strand, within BPHYT_RS34095at 3247.762 kb on - strand, within BPHYT_RS34095at 3248.321 kb on - strandat 3248.536 kb on - strand, within BPHYT_RS34100at 3248.794 kb on - strand, within BPHYT_RS34100

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Per-strain Table

Position Strand Gene LocusTag Fraction 24hr Phyto Lib Only
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3,246,487 + BPHYT_RS34090 0.52 +0.1
3,246,488 - BPHYT_RS34090 0.52 -1.6
3,246,885 - BPHYT_RS34090 0.83 -0.1
3,247,168 - +0.1
3,247,401 + BPHYT_RS34095 0.18 -1.1
3,247,440 + BPHYT_RS34095 0.21 -0.6
3,247,501 - BPHYT_RS34095 0.27 -1.2
3,247,570 - BPHYT_RS34095 0.34 +0.3
3,247,762 - BPHYT_RS34095 0.52 -0.6
3,247,762 - BPHYT_RS34095 0.52 -0.1
3,248,321 - +0.2
3,248,536 - BPHYT_RS34100 0.15 -0.9
3,248,794 - BPHYT_RS34100 0.38 -0.8

Or see this region's nucleotide sequence