Experiment: 24hr Phyto Lib Only
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS20965 and BPHYT_RS20970 are separated by 70 nucleotides BPHYT_RS20970 and BPHYT_RS20975 are separated by 138 nucleotides BPHYT_RS20975 and BPHYT_RS20980 are separated by 692 nucleotides
BPHYT_RS20965: BPHYT_RS20965 - photosystem reaction center subunit H, at 263,954 to 264,427
_RS20965
BPHYT_RS20970: BPHYT_RS20970 - hypothetical protein, at 264,498 to 264,728
_RS20970
BPHYT_RS20975: BPHYT_RS20975 - haloacid dehalogenase, at 264,867 to 265,595
_RS20975
BPHYT_RS20980: BPHYT_RS20980 - hypothetical protein, at 266,288 to 266,614
_RS20980
Position (kb) 264
265
266 Strain fitness (log2 ratio) -2
-1
0
1 at 263.943 kb on - strand at 263.946 kb on - strand at 264.031 kb on - strand, within BPHYT_RS20965 at 264.452 kb on - strand at 264.659 kb on + strand, within BPHYT_RS20970 at 264.855 kb on + strand at 265.096 kb on + strand, within BPHYT_RS20975 at 265.096 kb on + strand, within BPHYT_RS20975 at 265.417 kb on - strand, within BPHYT_RS20975 at 265.696 kb on + strand at 265.824 kb on + strand at 265.827 kb on + strand at 265.828 kb on - strand at 265.887 kb on + strand at 265.959 kb on + strand at 265.963 kb on + strand at 265.964 kb on - strand at 265.964 kb on - strand at 265.964 kb on - strand at 266.001 kb on + strand at 266.022 kb on + strand at 266.022 kb on + strand at 266.275 kb on - strand at 266.580 kb on + strand, within BPHYT_RS20980
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr Phyto Lib Only remove 263,943 - -0.5 263,946 - -0.6 264,031 - BPHYT_RS20965 0.16 -0.4 264,452 - -0.1 264,659 + BPHYT_RS20970 0.70 -0.6 264,855 + +0.7 265,096 + BPHYT_RS20975 0.31 -1.4 265,096 + BPHYT_RS20975 0.31 -0.4 265,417 - BPHYT_RS20975 0.75 +0.2 265,696 + -0.2 265,824 + +0.7 265,827 + +0.0 265,828 - -0.8 265,887 + -2.2 265,959 + -1.1 265,963 + +1.1 265,964 - +0.3 265,964 - +0.5 265,964 - -0.5 266,001 + -1.3 266,022 + -0.3 266,022 + -0.1 266,275 - +0.4 266,580 + BPHYT_RS20980 0.89 +1.5
Or see this region's nucleotide sequence