Experiment: 24hr Phyto Lib Only
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS13625 and BPHYT_RS13630 are separated by 158 nucleotides BPHYT_RS13630 and BPHYT_RS13635 are separated by 29 nucleotides BPHYT_RS13635 and BPHYT_RS13640 are separated by 246 nucleotides BPHYT_RS13640 and BPHYT_RS13645 are separated by 103 nucleotides
BPHYT_RS13625: BPHYT_RS13625 - phenylalanine--tRNA ligase alpha subunit, at 3,106,841 to 3,107,854
_RS13625
BPHYT_RS13630: BPHYT_RS13630 - 50S ribosomal protein L20, at 3,108,013 to 3,108,372
_RS13630
BPHYT_RS13635: BPHYT_RS13635 - 50S ribosomal protein L35, at 3,108,402 to 3,108,599
_RS13635
BPHYT_RS13640: BPHYT_RS13640 - translation initiation factor IF-3, at 3,108,846 to 3,109,316
_RS13640
BPHYT_RS13645: BPHYT_RS13645 - threonine--tRNA ligase, at 3,109,420 to 3,111,327
_RS13645
Position (kb)
3108
3109 Strain fitness (log2 ratio)
-1
0
1
2
3 at 3108.735 kb on - strand at 3108.735 kb on - strand at 3108.735 kb on - strand at 3109.557 kb on - strand at 3109.591 kb on - strand at 3109.592 kb on + strand at 3109.593 kb on - strand at 3109.593 kb on - strand at 3109.593 kb on - strand at 3109.597 kb on - strand at 3109.597 kb on - strand at 3109.597 kb on - strand at 3109.597 kb on - strand at 3109.597 kb on - strand at 3109.597 kb on - strand at 3109.597 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr Phyto Lib Only remove 3,108,735 - -1.2 3,108,735 - -0.7 3,108,735 - +0.1 3,109,557 - +2.9 3,109,591 - +3.2 3,109,592 + +2.9 3,109,593 - +2.0 3,109,593 - +2.1 3,109,593 - +2.8 3,109,597 - +2.9 3,109,597 - +2.8 3,109,597 - +2.8 3,109,597 - +2.8 3,109,597 - +2.8 3,109,597 - +3.4 3,109,597 - +2.5
Or see this region's nucleotide sequence