Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS11495

Experiment: 24hr Phyto Lib Only

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS11485 and BPHYT_RS11490 are separated by 171 nucleotidesBPHYT_RS11490 and BPHYT_RS11495 are separated by 133 nucleotidesBPHYT_RS11495 and BPHYT_RS11500 are separated by 53 nucleotides BPHYT_RS11485: BPHYT_RS11485 - 6-phosphogluconate dehydrogenase, at 2,612,309 to 2,613,376 _RS11485 BPHYT_RS11490: BPHYT_RS11490 - hypothetical protein, at 2,613,548 to 2,613,754 _RS11490 BPHYT_RS11495: BPHYT_RS11495 - membrane protein, at 2,613,888 to 2,614,994 _RS11495 BPHYT_RS11500: BPHYT_RS11500 - ABC transporter permease, at 2,615,048 to 2,615,977 _RS11500 Position (kb) 2613 2614 2615Strain fitness (log2 ratio) -1 0 1 2at 2612.948 kb on + strand, within BPHYT_RS11485at 2613.167 kb on + strand, within BPHYT_RS11485at 2613.443 kb on + strandat 2613.509 kb on + strandat 2613.563 kb on + strandat 2613.563 kb on + strandat 2613.922 kb on + strandat 2613.923 kb on - strandat 2613.923 kb on - strandat 2614.193 kb on - strand, within BPHYT_RS11495at 2614.193 kb on - strand, within BPHYT_RS11495at 2614.610 kb on - strand, within BPHYT_RS11495at 2615.152 kb on - strand, within BPHYT_RS11500at 2615.644 kb on - strand, within BPHYT_RS11500at 2615.659 kb on - strand, within BPHYT_RS11500

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Per-strain Table

Position Strand Gene LocusTag Fraction 24hr Phyto Lib Only
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2,612,948 + BPHYT_RS11485 0.60 +0.2
2,613,167 + BPHYT_RS11485 0.80 -0.1
2,613,443 + -0.8
2,613,509 + -0.2
2,613,563 + -0.1
2,613,563 + -0.7
2,613,922 + -0.2
2,613,923 - +0.1
2,613,923 - +0.3
2,614,193 - BPHYT_RS11495 0.28 -0.3
2,614,193 - BPHYT_RS11495 0.28 +1.8
2,614,610 - BPHYT_RS11495 0.65 +0.0
2,615,152 - BPHYT_RS11500 0.11 -0.3
2,615,644 - BPHYT_RS11500 0.64 -0.7
2,615,659 - BPHYT_RS11500 0.66 +0.2

Or see this region's nucleotide sequence