Experiment: 24hr Phyto Lib Only
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS03225 and BPHYT_RS03230 are separated by 189 nucleotides BPHYT_RS03230 and BPHYT_RS03235 are separated by 118 nucleotides
BPHYT_RS03225: BPHYT_RS03225 - 3-hydroxyacyl-CoA dehydrogenase, at 723,469 to 725,904
_RS03225
BPHYT_RS03230: BPHYT_RS03230 - acetyl-CoA acetyltransferase, at 726,094 to 727,293
_RS03230
BPHYT_RS03235: BPHYT_RS03235 - enoyl-CoA hydratase, at 727,412 to 728,182
_RS03235
Position (kb) 726
727
728 Strain fitness (log2 ratio) -1
0
1 at 725.221 kb on + strand, within BPHYT_RS03225 at 725.902 kb on + strand at 725.923 kb on + strand at 725.997 kb on + strand at 726.012 kb on + strand at 726.118 kb on + strand at 726.118 kb on + strand at 726.118 kb on + strand at 726.119 kb on - strand at 726.119 kb on - strand at 726.138 kb on + strand at 726.139 kb on - strand at 726.346 kb on - strand, within BPHYT_RS03230 at 726.492 kb on + strand, within BPHYT_RS03230 at 726.492 kb on + strand, within BPHYT_RS03230 at 726.493 kb on - strand, within BPHYT_RS03230 at 726.513 kb on + strand, within BPHYT_RS03230 at 726.548 kb on - strand, within BPHYT_RS03230 at 726.849 kb on + strand, within BPHYT_RS03230 at 726.850 kb on - strand, within BPHYT_RS03230 at 726.850 kb on - strand, within BPHYT_RS03230 at 726.850 kb on - strand, within BPHYT_RS03230 at 727.228 kb on + strand at 727.228 kb on + strand at 727.229 kb on - strand at 727.261 kb on - strand at 727.423 kb on + strand at 727.493 kb on - strand, within BPHYT_RS03235 at 727.505 kb on + strand, within BPHYT_RS03235 at 727.505 kb on + strand, within BPHYT_RS03235 at 728.205 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr Phyto Lib Only remove 725,221 + BPHYT_RS03225 0.72 +0.1 725,902 + -1.1 725,923 + -0.6 725,997 + -0.7 726,012 + -1.0 726,118 + -0.9 726,118 + +0.5 726,118 + +1.1 726,119 - -0.1 726,119 - -1.2 726,138 + -1.0 726,139 - -0.1 726,346 - BPHYT_RS03230 0.21 -0.5 726,492 + BPHYT_RS03230 0.33 +0.9 726,492 + BPHYT_RS03230 0.33 -0.8 726,493 - BPHYT_RS03230 0.33 -0.2 726,513 + BPHYT_RS03230 0.35 +1.3 726,548 - BPHYT_RS03230 0.38 +0.6 726,849 + BPHYT_RS03230 0.63 -0.3 726,850 - BPHYT_RS03230 0.63 -0.7 726,850 - BPHYT_RS03230 0.63 -0.1 726,850 - BPHYT_RS03230 0.63 +0.4 727,228 + +0.2 727,228 + -0.6 727,229 - -1.3 727,261 - -0.2 727,423 + -0.5 727,493 - BPHYT_RS03235 0.11 -1.3 727,505 + BPHYT_RS03235 0.12 -0.4 727,505 + BPHYT_RS03235 0.12 +0.5 728,205 + +0.6
Or see this region's nucleotide sequence