Experiment: 24hr PHYTO6 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS20880 and BPHYT_RS20885 are separated by 187 nucleotides BPHYT_RS20885 and BPHYT_RS20890 are separated by 94 nucleotides BPHYT_RS20890 and BPHYT_RS20895 are separated by 345 nucleotides
BPHYT_RS20880: BPHYT_RS20880 - polyketide cyclase, at 245,946 to 246,491
_RS20880
BPHYT_RS20885: BPHYT_RS20885 - nuclear protein SET, at 246,679 to 247,119
_RS20885
BPHYT_RS20890: BPHYT_RS20890 - transglycosylase, at 247,214 to 247,465
_RS20890
BPHYT_RS20895: BPHYT_RS20895 - ABC transporter, at 247,811 to 249,604
_RS20895
Position (kb) 247
248 Strain fitness (log2 ratio) -2
-1
0
1
2 at 246.635 kb on - strand at 246.678 kb on + strand at 246.695 kb on + strand at 247.005 kb on + strand, within BPHYT_RS20885 at 247.013 kb on - strand, within BPHYT_RS20885 at 247.060 kb on + strand, within BPHYT_RS20885 at 247.122 kb on + strand at 247.271 kb on - strand, within BPHYT_RS20890 at 247.372 kb on + strand, within BPHYT_RS20890 at 247.575 kb on + strand at 247.575 kb on + strand at 247.575 kb on + strand at 247.576 kb on - strand at 247.664 kb on - strand at 247.787 kb on + strand at 247.970 kb on + strand at 248.035 kb on - strand, within BPHYT_RS20895 at 248.193 kb on + strand, within BPHYT_RS20895 at 248.194 kb on - strand, within BPHYT_RS20895 at 248.365 kb on + strand, within BPHYT_RS20895 at 248.366 kb on - strand, within BPHYT_RS20895
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO6 MOI 0.1 remove 246,635 - -1.3 246,678 + +1.0 246,695 + -1.1 247,005 + BPHYT_RS20885 0.74 +0.4 247,013 - BPHYT_RS20885 0.76 -2.0 247,060 + BPHYT_RS20885 0.86 -0.5 247,122 + -0.3 247,271 - BPHYT_RS20890 0.23 +0.3 247,372 + BPHYT_RS20890 0.63 +0.5 247,575 + +0.1 247,575 + -1.4 247,575 + +1.4 247,576 - -0.1 247,664 - -1.1 247,787 + -2.0 247,970 + +0.1 248,035 - BPHYT_RS20895 0.12 +2.4 248,193 + BPHYT_RS20895 0.21 -0.8 248,194 - BPHYT_RS20895 0.21 -1.2 248,365 + BPHYT_RS20895 0.31 -2.2 248,366 - BPHYT_RS20895 0.31 +1.5
Or see this region's nucleotide sequence