Experiment: 24hr PHYTO6 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS01865 and BPHYT_RS01870 are separated by 108 nucleotides BPHYT_RS01870 and BPHYT_RS01875 are separated by 284 nucleotides
BPHYT_RS01865: BPHYT_RS01865 - major facilitator transporter, at 423,297 to 424,589
_RS01865
BPHYT_RS01870: BPHYT_RS01870 - 6-aminohexanoate-cyclic-dimer hydrolase, at 424,698 to 426,218
_RS01870
BPHYT_RS01875: BPHYT_RS01875 - carboxymethylenebutenolidase, at 426,503 to 427,201
_RS01875
Position (kb) 424
425
426
427 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 423.900 kb on + strand, within BPHYT_RS01865 at 423.901 kb on - strand, within BPHYT_RS01865 at 424.047 kb on + strand, within BPHYT_RS01865 at 424.047 kb on + strand, within BPHYT_RS01865 at 424.048 kb on - strand, within BPHYT_RS01865 at 424.063 kb on - strand, within BPHYT_RS01865 at 424.063 kb on - strand, within BPHYT_RS01865 at 424.101 kb on + strand, within BPHYT_RS01865 at 424.223 kb on + strand, within BPHYT_RS01865 at 424.224 kb on - strand, within BPHYT_RS01865 at 424.239 kb on + strand, within BPHYT_RS01865 at 424.240 kb on - strand, within BPHYT_RS01865 at 424.314 kb on - strand, within BPHYT_RS01865 at 424.327 kb on - strand, within BPHYT_RS01865 at 424.329 kb on + strand, within BPHYT_RS01865 at 424.401 kb on + strand, within BPHYT_RS01865 at 424.402 kb on - strand, within BPHYT_RS01865 at 424.491 kb on + strand at 424.492 kb on - strand at 424.492 kb on - strand at 424.495 kb on - strand at 424.699 kb on + strand at 424.700 kb on - strand at 424.700 kb on - strand at 424.844 kb on - strand at 425.321 kb on + strand, within BPHYT_RS01870 at 425.321 kb on + strand, within BPHYT_RS01870 at 425.322 kb on - strand, within BPHYT_RS01870 at 425.322 kb on - strand, within BPHYT_RS01870 at 425.413 kb on + strand, within BPHYT_RS01870 at 425.420 kb on + strand, within BPHYT_RS01870 at 425.694 kb on - strand, within BPHYT_RS01870 at 425.792 kb on + strand, within BPHYT_RS01870 at 425.792 kb on + strand, within BPHYT_RS01870 at 425.793 kb on - strand, within BPHYT_RS01870 at 425.794 kb on + strand, within BPHYT_RS01870 at 425.794 kb on + strand, within BPHYT_RS01870 at 425.795 kb on - strand, within BPHYT_RS01870 at 426.070 kb on + strand at 426.168 kb on + strand at 426.169 kb on - strand at 426.361 kb on + strand at 426.370 kb on + strand at 426.370 kb on + strand at 426.371 kb on - strand at 426.504 kb on + strand at 426.567 kb on + strand at 426.567 kb on + strand at 426.568 kb on - strand at 426.612 kb on + strand, within BPHYT_RS01875 at 426.613 kb on - strand, within BPHYT_RS01875 at 426.772 kb on - strand, within BPHYT_RS01875 at 426.772 kb on - strand, within BPHYT_RS01875 at 426.813 kb on + strand, within BPHYT_RS01875 at 426.813 kb on + strand, within BPHYT_RS01875 at 426.975 kb on + strand, within BPHYT_RS01875 at 426.975 kb on + strand, within BPHYT_RS01875 at 427.070 kb on - strand, within BPHYT_RS01875 at 427.144 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO6 MOI 0.1 remove 423,900 + BPHYT_RS01865 0.47 -0.2 423,901 - BPHYT_RS01865 0.47 -1.4 424,047 + BPHYT_RS01865 0.58 -1.1 424,047 + BPHYT_RS01865 0.58 +0.5 424,048 - BPHYT_RS01865 0.58 -0.0 424,063 - BPHYT_RS01865 0.59 +3.0 424,063 - BPHYT_RS01865 0.59 -1.2 424,101 + BPHYT_RS01865 0.62 +1.6 424,223 + BPHYT_RS01865 0.72 -0.5 424,224 - BPHYT_RS01865 0.72 +0.2 424,239 + BPHYT_RS01865 0.73 -0.3 424,240 - BPHYT_RS01865 0.73 +0.1 424,314 - BPHYT_RS01865 0.79 -0.3 424,327 - BPHYT_RS01865 0.80 -0.5 424,329 + BPHYT_RS01865 0.80 -3.3 424,401 + BPHYT_RS01865 0.85 -0.5 424,402 - BPHYT_RS01865 0.85 -0.7 424,491 + -0.6 424,492 - +0.0 424,492 - -1.0 424,495 - -0.4 424,699 + +0.0 424,700 - -0.3 424,700 - -0.7 424,844 - -0.5 425,321 + BPHYT_RS01870 0.41 -0.4 425,321 + BPHYT_RS01870 0.41 -0.5 425,322 - BPHYT_RS01870 0.41 -1.4 425,322 - BPHYT_RS01870 0.41 -0.2 425,413 + BPHYT_RS01870 0.47 +0.6 425,420 + BPHYT_RS01870 0.47 -0.0 425,694 - BPHYT_RS01870 0.65 -0.9 425,792 + BPHYT_RS01870 0.72 -0.1 425,792 + BPHYT_RS01870 0.72 -1.1 425,793 - BPHYT_RS01870 0.72 +0.2 425,794 + BPHYT_RS01870 0.72 +0.4 425,794 + BPHYT_RS01870 0.72 -0.0 425,795 - BPHYT_RS01870 0.72 +0.9 426,070 + +0.4 426,168 + +0.1 426,169 - +0.6 426,361 + +1.2 426,370 + -0.2 426,370 + +0.5 426,371 - -2.0 426,504 + -1.0 426,567 + +0.3 426,567 + -0.3 426,568 - -0.5 426,612 + BPHYT_RS01875 0.16 -0.7 426,613 - BPHYT_RS01875 0.16 -1.0 426,772 - BPHYT_RS01875 0.38 -1.2 426,772 - BPHYT_RS01875 0.38 -0.4 426,813 + BPHYT_RS01875 0.44 +0.6 426,813 + BPHYT_RS01875 0.44 -0.1 426,975 + BPHYT_RS01875 0.68 +0.6 426,975 + BPHYT_RS01875 0.68 -1.5 427,070 - BPHYT_RS01875 0.81 -0.6 427,144 - -0.6
Or see this region's nucleotide sequence