Experiment: 24hr PHYTO6 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS31670 and BPHYT_RS31675 are separated by 298 nucleotides BPHYT_RS31675 and BPHYT_RS31680 are separated by 129 nucleotides BPHYT_RS31680 and BPHYT_RS31685 are separated by 57 nucleotides
BPHYT_RS31670: BPHYT_RS31670 - transposase, at 2,661,861 to 2,662,196
_RS31670
BPHYT_RS31675: BPHYT_RS31675 - hypothetical protein, at 2,662,495 to 2,662,821
_RS31675
BPHYT_RS31680: BPHYT_RS31680 - dehydrogenase, at 2,662,951 to 2,663,844
_RS31680
BPHYT_RS31685: BPHYT_RS31685 - D-isomer specific 2-hydroxyacid dehydrogenase, at 2,663,902 to 2,664,882
_RS31685
Position (kb)
2662
2663
2664 Strain fitness (log2 ratio)
-1
0
1 at 2662.153 kb on - strand, within BPHYT_RS31670 at 2662.227 kb on - strand at 2662.554 kb on + strand, within BPHYT_RS31675 at 2662.696 kb on - strand, within BPHYT_RS31675 at 2662.923 kb on - strand at 2662.938 kb on - strand at 2662.940 kb on + strand at 2662.944 kb on + strand at 2663.263 kb on - strand, within BPHYT_RS31680 at 2663.354 kb on + strand, within BPHYT_RS31680 at 2663.378 kb on + strand, within BPHYT_RS31680 at 2663.527 kb on - strand, within BPHYT_RS31680 at 2663.647 kb on - strand, within BPHYT_RS31680 at 2663.844 kb on + strand at 2664.285 kb on - strand, within BPHYT_RS31685 at 2664.307 kb on - strand, within BPHYT_RS31685 at 2664.582 kb on + strand, within BPHYT_RS31685
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO6 MOI 0.1 remove 2,662,153 - BPHYT_RS31670 0.87 -0.8 2,662,227 - +0.6 2,662,554 + BPHYT_RS31675 0.18 -1.1 2,662,696 - BPHYT_RS31675 0.61 -0.4 2,662,923 - +0.6 2,662,938 - -0.6 2,662,940 + +1.4 2,662,944 + +0.4 2,663,263 - BPHYT_RS31680 0.35 -0.3 2,663,354 + BPHYT_RS31680 0.45 -1.5 2,663,378 + BPHYT_RS31680 0.48 -1.5 2,663,527 - BPHYT_RS31680 0.64 -0.0 2,663,647 - BPHYT_RS31680 0.78 -0.1 2,663,844 + +0.2 2,664,285 - BPHYT_RS31685 0.39 +1.0 2,664,307 - BPHYT_RS31685 0.41 -1.6 2,664,582 + BPHYT_RS31685 0.69 +1.6
Or see this region's nucleotide sequence