Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS12305

Experiment: 24hr PHYTO6 MOI 0.1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS12300 and BPHYT_RS12305 are separated by 83 nucleotidesBPHYT_RS12305 and BPHYT_RS12310 are separated by 174 nucleotidesBPHYT_RS12310 and BPHYT_RS12315 are separated by 94 nucleotides BPHYT_RS12300: BPHYT_RS12300 - haloacid dehalogenase, at 2,809,379 to 2,810,194 _RS12300 BPHYT_RS12305: BPHYT_RS12305 - Malate synthase (EC 2.3.3.9) (from data), at 2,810,278 to 2,811,888 _RS12305 BPHYT_RS12310: BPHYT_RS12310 - gamma-glutamyl cyclotransferase, at 2,812,063 to 2,812,464 _RS12310 BPHYT_RS12315: BPHYT_RS12315 - ribonuclease, at 2,812,559 to 2,813,053 _RS12315 Position (kb) 2810 2811 2812Strain fitness (log2 ratio) -2 -1 0 1at 2809.278 kb on + strandat 2809.339 kb on + strandat 2809.532 kb on + strand, within BPHYT_RS12300at 2809.533 kb on - strand, within BPHYT_RS12300at 2809.533 kb on - strand, within BPHYT_RS12300at 2809.533 kb on - strand, within BPHYT_RS12300at 2809.685 kb on + strand, within BPHYT_RS12300at 2809.881 kb on + strand, within BPHYT_RS12300at 2809.881 kb on + strand, within BPHYT_RS12300at 2809.881 kb on + strand, within BPHYT_RS12300at 2809.882 kb on - strand, within BPHYT_RS12300at 2809.925 kb on + strand, within BPHYT_RS12300at 2810.676 kb on + strand, within BPHYT_RS12305at 2810.695 kb on - strand, within BPHYT_RS12305at 2810.752 kb on + strand, within BPHYT_RS12305at 2810.752 kb on + strand, within BPHYT_RS12305at 2810.753 kb on - strand, within BPHYT_RS12305at 2810.920 kb on + strand, within BPHYT_RS12305at 2810.921 kb on - strand, within BPHYT_RS12305at 2810.921 kb on - strand, within BPHYT_RS12305at 2811.096 kb on + strand, within BPHYT_RS12305at 2811.096 kb on + strand, within BPHYT_RS12305at 2811.096 kb on + strand, within BPHYT_RS12305at 2811.485 kb on + strand, within BPHYT_RS12305at 2811.485 kb on + strand, within BPHYT_RS12305at 2811.578 kb on - strand, within BPHYT_RS12305at 2811.578 kb on - strand, within BPHYT_RS12305at 2811.578 kb on - strand, within BPHYT_RS12305at 2811.800 kb on - strandat 2811.800 kb on - strandat 2811.959 kb on + strandat 2811.960 kb on - strandat 2812.021 kb on - strandat 2812.021 kb on - strandat 2812.021 kb on - strandat 2812.448 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 24hr PHYTO6 MOI 0.1
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2,809,278 + -1.1
2,809,339 + +0.4
2,809,532 + BPHYT_RS12300 0.19 -0.8
2,809,533 - BPHYT_RS12300 0.19 -0.4
2,809,533 - BPHYT_RS12300 0.19 +0.2
2,809,533 - BPHYT_RS12300 0.19 -0.0
2,809,685 + BPHYT_RS12300 0.38 -2.0
2,809,881 + BPHYT_RS12300 0.62 +0.8
2,809,881 + BPHYT_RS12300 0.62 +0.2
2,809,881 + BPHYT_RS12300 0.62 +0.3
2,809,882 - BPHYT_RS12300 0.62 -0.0
2,809,925 + BPHYT_RS12300 0.67 -0.1
2,810,676 + BPHYT_RS12305 0.25 -1.2
2,810,695 - BPHYT_RS12305 0.26 -1.9
2,810,752 + BPHYT_RS12305 0.29 -0.9
2,810,752 + BPHYT_RS12305 0.29 -0.9
2,810,753 - BPHYT_RS12305 0.29 -0.7
2,810,920 + BPHYT_RS12305 0.40 -0.8
2,810,921 - BPHYT_RS12305 0.40 -0.3
2,810,921 - BPHYT_RS12305 0.40 -0.5
2,811,096 + BPHYT_RS12305 0.51 -0.7
2,811,096 + BPHYT_RS12305 0.51 +0.4
2,811,096 + BPHYT_RS12305 0.51 -0.1
2,811,485 + BPHYT_RS12305 0.75 +0.6
2,811,485 + BPHYT_RS12305 0.75 -0.5
2,811,578 - BPHYT_RS12305 0.81 +0.0
2,811,578 - BPHYT_RS12305 0.81 -1.3
2,811,578 - BPHYT_RS12305 0.81 -1.1
2,811,800 - -1.3
2,811,800 - +0.1
2,811,959 + -1.2
2,811,960 - -2.1
2,812,021 - +0.2
2,812,021 - +0.2
2,812,021 - -0.1
2,812,448 - -0.9

Or see this region's nucleotide sequence