Experiment: 24hr PHYTO6 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS12300 and BPHYT_RS12305 are separated by 83 nucleotides BPHYT_RS12305 and BPHYT_RS12310 are separated by 174 nucleotides BPHYT_RS12310 and BPHYT_RS12315 are separated by 94 nucleotides
BPHYT_RS12300: BPHYT_RS12300 - haloacid dehalogenase, at 2,809,379 to 2,810,194
_RS12300
BPHYT_RS12305: BPHYT_RS12305 - Malate synthase (EC 2.3.3.9) (from data) , at 2,810,278 to 2,811,888
_RS12305
BPHYT_RS12310: BPHYT_RS12310 - gamma-glutamyl cyclotransferase, at 2,812,063 to 2,812,464
_RS12310
BPHYT_RS12315: BPHYT_RS12315 - ribonuclease, at 2,812,559 to 2,813,053
_RS12315
Position (kb)
2810
2811
2812 Strain fitness (log2 ratio)
-2
-1
0
1 at 2809.278 kb on + strand at 2809.339 kb on + strand at 2809.532 kb on + strand, within BPHYT_RS12300 at 2809.533 kb on - strand, within BPHYT_RS12300 at 2809.533 kb on - strand, within BPHYT_RS12300 at 2809.533 kb on - strand, within BPHYT_RS12300 at 2809.685 kb on + strand, within BPHYT_RS12300 at 2809.881 kb on + strand, within BPHYT_RS12300 at 2809.881 kb on + strand, within BPHYT_RS12300 at 2809.881 kb on + strand, within BPHYT_RS12300 at 2809.882 kb on - strand, within BPHYT_RS12300 at 2809.925 kb on + strand, within BPHYT_RS12300 at 2810.676 kb on + strand, within BPHYT_RS12305 at 2810.695 kb on - strand, within BPHYT_RS12305 at 2810.752 kb on + strand, within BPHYT_RS12305 at 2810.752 kb on + strand, within BPHYT_RS12305 at 2810.753 kb on - strand, within BPHYT_RS12305 at 2810.920 kb on + strand, within BPHYT_RS12305 at 2810.921 kb on - strand, within BPHYT_RS12305 at 2810.921 kb on - strand, within BPHYT_RS12305 at 2811.096 kb on + strand, within BPHYT_RS12305 at 2811.096 kb on + strand, within BPHYT_RS12305 at 2811.096 kb on + strand, within BPHYT_RS12305 at 2811.485 kb on + strand, within BPHYT_RS12305 at 2811.485 kb on + strand, within BPHYT_RS12305 at 2811.578 kb on - strand, within BPHYT_RS12305 at 2811.578 kb on - strand, within BPHYT_RS12305 at 2811.578 kb on - strand, within BPHYT_RS12305 at 2811.800 kb on - strand at 2811.800 kb on - strand at 2811.959 kb on + strand at 2811.960 kb on - strand at 2812.021 kb on - strand at 2812.021 kb on - strand at 2812.021 kb on - strand at 2812.448 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO6 MOI 0.1 remove 2,809,278 + -1.1 2,809,339 + +0.4 2,809,532 + BPHYT_RS12300 0.19 -0.8 2,809,533 - BPHYT_RS12300 0.19 -0.4 2,809,533 - BPHYT_RS12300 0.19 +0.2 2,809,533 - BPHYT_RS12300 0.19 -0.0 2,809,685 + BPHYT_RS12300 0.38 -2.0 2,809,881 + BPHYT_RS12300 0.62 +0.8 2,809,881 + BPHYT_RS12300 0.62 +0.2 2,809,881 + BPHYT_RS12300 0.62 +0.3 2,809,882 - BPHYT_RS12300 0.62 -0.0 2,809,925 + BPHYT_RS12300 0.67 -0.1 2,810,676 + BPHYT_RS12305 0.25 -1.2 2,810,695 - BPHYT_RS12305 0.26 -1.9 2,810,752 + BPHYT_RS12305 0.29 -0.9 2,810,752 + BPHYT_RS12305 0.29 -0.9 2,810,753 - BPHYT_RS12305 0.29 -0.7 2,810,920 + BPHYT_RS12305 0.40 -0.8 2,810,921 - BPHYT_RS12305 0.40 -0.3 2,810,921 - BPHYT_RS12305 0.40 -0.5 2,811,096 + BPHYT_RS12305 0.51 -0.7 2,811,096 + BPHYT_RS12305 0.51 +0.4 2,811,096 + BPHYT_RS12305 0.51 -0.1 2,811,485 + BPHYT_RS12305 0.75 +0.6 2,811,485 + BPHYT_RS12305 0.75 -0.5 2,811,578 - BPHYT_RS12305 0.81 +0.0 2,811,578 - BPHYT_RS12305 0.81 -1.3 2,811,578 - BPHYT_RS12305 0.81 -1.1 2,811,800 - -1.3 2,811,800 - +0.1 2,811,959 + -1.2 2,811,960 - -2.1 2,812,021 - +0.2 2,812,021 - +0.2 2,812,021 - -0.1 2,812,448 - -0.9
Or see this region's nucleotide sequence