Experiment: 24hr PHYTO6 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS05810 and BPHYT_RS05815 are separated by 35 nucleotides BPHYT_RS05815 and BPHYT_RS05820 overlap by 4 nucleotides BPHYT_RS05820 and BPHYT_RS05825 are separated by 32 nucleotides BPHYT_RS05825 and BPHYT_RS05830 are separated by 165 nucleotides BPHYT_RS05830 and BPHYT_RS05835 are separated by 32 nucleotides
BPHYT_RS05810: BPHYT_RS05810 - hypothetical protein, at 1,323,255 to 1,324,721
_RS05810
BPHYT_RS05815: BPHYT_RS05815 - hypothetical protein, at 1,324,757 to 1,325,635
_RS05815
BPHYT_RS05820: BPHYT_RS05820 - hypothetical protein, at 1,325,632 to 1,327,530
_RS05820
BPHYT_RS05825: BPHYT_RS05825 - hypothetical protein, at 1,327,563 to 1,327,784
_RS05825
BPHYT_RS05830: BPHYT_RS05830 - hypothetical protein, at 1,327,950 to 1,328,408
_RS05830
BPHYT_RS05835: BPHYT_RS05835 - hypothetical protein, at 1,328,441 to 1,328,845
_RS05835
Position (kb)
1325
1326
1327
1328 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1324.677 kb on - strand at 1324.729 kb on + strand at 1324.898 kb on - strand, within BPHYT_RS05815 at 1325.166 kb on - strand, within BPHYT_RS05815 at 1325.181 kb on + strand, within BPHYT_RS05815 at 1325.256 kb on - strand, within BPHYT_RS05815 at 1325.316 kb on - strand, within BPHYT_RS05815 at 1325.685 kb on + strand at 1325.715 kb on + strand at 1325.716 kb on - strand at 1325.716 kb on - strand at 1325.920 kb on - strand, within BPHYT_RS05820 at 1326.004 kb on + strand, within BPHYT_RS05820 at 1326.220 kb on - strand, within BPHYT_RS05820 at 1326.308 kb on + strand, within BPHYT_RS05820 at 1326.308 kb on + strand, within BPHYT_RS05820 at 1326.308 kb on + strand, within BPHYT_RS05820 at 1326.309 kb on - strand, within BPHYT_RS05820 at 1326.432 kb on - strand, within BPHYT_RS05820 at 1326.559 kb on + strand, within BPHYT_RS05820 at 1326.762 kb on + strand, within BPHYT_RS05820 at 1326.853 kb on - strand, within BPHYT_RS05820 at 1326.898 kb on + strand, within BPHYT_RS05820 at 1326.931 kb on - strand, within BPHYT_RS05820 at 1326.931 kb on - strand, within BPHYT_RS05820 at 1326.951 kb on + strand, within BPHYT_RS05820 at 1326.954 kb on + strand, within BPHYT_RS05820 at 1326.955 kb on - strand, within BPHYT_RS05820 at 1327.013 kb on - strand, within BPHYT_RS05820 at 1327.020 kb on + strand, within BPHYT_RS05820 at 1327.020 kb on + strand, within BPHYT_RS05820 at 1327.021 kb on - strand, within BPHYT_RS05820 at 1327.096 kb on - strand, within BPHYT_RS05820 at 1327.147 kb on + strand, within BPHYT_RS05820 at 1327.147 kb on + strand, within BPHYT_RS05820 at 1327.183 kb on + strand, within BPHYT_RS05820 at 1327.227 kb on - strand, within BPHYT_RS05820 at 1327.314 kb on + strand, within BPHYT_RS05820 at 1327.315 kb on - strand, within BPHYT_RS05820 at 1327.368 kb on + strand at 1327.490 kb on - strand at 1327.519 kb on + strand at 1327.693 kb on + strand, within BPHYT_RS05825 at 1327.694 kb on - strand, within BPHYT_RS05825 at 1327.898 kb on + strand at 1328.009 kb on - strand, within BPHYT_RS05830 at 1328.223 kb on + strand, within BPHYT_RS05830 at 1328.245 kb on + strand, within BPHYT_RS05830 at 1328.375 kb on - strand at 1328.471 kb on + strand at 1328.471 kb on + strand at 1328.471 kb on + strand at 1328.471 kb on + strand at 1328.472 kb on - strand at 1328.478 kb on - strand at 1328.489 kb on + strand, within BPHYT_RS05835
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO6 MOI 0.1 remove 1,324,677 - +0.3 1,324,729 + +2.3 1,324,898 - BPHYT_RS05815 0.16 +0.3 1,325,166 - BPHYT_RS05815 0.47 -0.4 1,325,181 + BPHYT_RS05815 0.48 -0.8 1,325,256 - BPHYT_RS05815 0.57 +0.3 1,325,316 - BPHYT_RS05815 0.64 -0.1 1,325,685 + -0.4 1,325,715 + +0.7 1,325,716 - +0.0 1,325,716 - +1.0 1,325,920 - BPHYT_RS05820 0.15 -1.2 1,326,004 + BPHYT_RS05820 0.20 -0.1 1,326,220 - BPHYT_RS05820 0.31 -2.6 1,326,308 + BPHYT_RS05820 0.36 -0.4 1,326,308 + BPHYT_RS05820 0.36 -0.1 1,326,308 + BPHYT_RS05820 0.36 -0.3 1,326,309 - BPHYT_RS05820 0.36 -0.0 1,326,432 - BPHYT_RS05820 0.42 -0.2 1,326,559 + BPHYT_RS05820 0.49 +0.1 1,326,762 + BPHYT_RS05820 0.60 -0.1 1,326,853 - BPHYT_RS05820 0.64 +0.6 1,326,898 + BPHYT_RS05820 0.67 -0.5 1,326,931 - BPHYT_RS05820 0.68 -0.8 1,326,931 - BPHYT_RS05820 0.68 +0.3 1,326,951 + BPHYT_RS05820 0.69 +0.5 1,326,954 + BPHYT_RS05820 0.70 -0.9 1,326,955 - BPHYT_RS05820 0.70 +0.4 1,327,013 - BPHYT_RS05820 0.73 +0.4 1,327,020 + BPHYT_RS05820 0.73 -1.7 1,327,020 + BPHYT_RS05820 0.73 -0.4 1,327,021 - BPHYT_RS05820 0.73 -0.6 1,327,096 - BPHYT_RS05820 0.77 -0.2 1,327,147 + BPHYT_RS05820 0.80 -0.8 1,327,147 + BPHYT_RS05820 0.80 +3.3 1,327,183 + BPHYT_RS05820 0.82 -2.1 1,327,227 - BPHYT_RS05820 0.84 -0.7 1,327,314 + BPHYT_RS05820 0.89 -0.2 1,327,315 - BPHYT_RS05820 0.89 -0.9 1,327,368 + +0.6 1,327,490 - -1.0 1,327,519 + +0.2 1,327,693 + BPHYT_RS05825 0.59 -1.4 1,327,694 - BPHYT_RS05825 0.59 -1.2 1,327,898 + -0.5 1,328,009 - BPHYT_RS05830 0.13 -0.2 1,328,223 + BPHYT_RS05830 0.59 -1.1 1,328,245 + BPHYT_RS05830 0.64 -0.1 1,328,375 - +0.1 1,328,471 + -1.5 1,328,471 + +0.6 1,328,471 + -0.8 1,328,471 + +0.6 1,328,472 - -0.4 1,328,478 - +1.1 1,328,489 + BPHYT_RS05835 0.12 -0.3
Or see this region's nucleotide sequence