Experiment: 24hr PHYTO6 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS36025 and BPHYT_RS36030 are separated by 50 nucleotides BPHYT_RS36030 and BPHYT_RS36035 are separated by 44 nucleotides BPHYT_RS36035 and BPHYT_RS36040 are separated by 24 nucleotides BPHYT_RS36040 and BPHYT_RS36045 overlap by 4 nucleotides BPHYT_RS36045 and BPHYT_RS36050 are separated by 20 nucleotides
BPHYT_RS36025: BPHYT_RS36025 - hypothetical protein, at 34,722 to 35,090
_RS36025
BPHYT_RS36030: BPHYT_RS36030 - hypothetical protein, at 35,141 to 35,560
_RS36030
BPHYT_RS36035: BPHYT_RS36035 - hypothetical protein, at 35,605 to 36,003
_RS36035
BPHYT_RS36040: BPHYT_RS36040 - hypothetical protein, at 36,028 to 36,657
_RS36040
BPHYT_RS36045: BPHYT_RS36045 - hypothetical protein, at 36,654 to 36,911
_RS36045
BPHYT_RS36050: BPHYT_RS36050 - hypothetical protein, at 36,932 to 37,393
_RS36050
Position (kb)
35
36
37 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 34.731 kb on - strand at 34.880 kb on + strand, within BPHYT_RS36025 at 34.881 kb on - strand, within BPHYT_RS36025 at 34.881 kb on - strand, within BPHYT_RS36025 at 34.924 kb on - strand, within BPHYT_RS36025 at 35.013 kb on - strand, within BPHYT_RS36025 at 35.013 kb on - strand, within BPHYT_RS36025 at 35.362 kb on - strand, within BPHYT_RS36030 at 35.418 kb on - strand, within BPHYT_RS36030 at 35.692 kb on - strand, within BPHYT_RS36035 at 35.789 kb on + strand, within BPHYT_RS36035 at 36.250 kb on + strand, within BPHYT_RS36040 at 36.250 kb on + strand, within BPHYT_RS36040 at 36.250 kb on + strand, within BPHYT_RS36040 at 36.250 kb on + strand, within BPHYT_RS36040
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO6 MOI 1 remove 34,731 - -0.2 34,880 + BPHYT_RS36025 0.43 -0.8 34,881 - BPHYT_RS36025 0.43 -1.0 34,881 - BPHYT_RS36025 0.43 -1.5 34,924 - BPHYT_RS36025 0.55 -0.5 35,013 - BPHYT_RS36025 0.79 +2.0 35,013 - BPHYT_RS36025 0.79 -0.5 35,362 - BPHYT_RS36030 0.53 +0.3 35,418 - BPHYT_RS36030 0.66 -1.0 35,692 - BPHYT_RS36035 0.22 -2.5 35,789 + BPHYT_RS36035 0.46 +0.4 36,250 + BPHYT_RS36040 0.35 +1.8 36,250 + BPHYT_RS36040 0.35 -1.3 36,250 + BPHYT_RS36040 0.35 -0.5 36,250 + BPHYT_RS36040 0.35 -1.4
Or see this region's nucleotide sequence