Experiment: 24hr PHYTO6 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS35385 and BPHYT_RS35390 are separated by 150 nucleotides BPHYT_RS35390 and BPHYT_RS35395 are separated by 14 nucleotides BPHYT_RS35395 and BPHYT_RS35400 are separated by 4 nucleotides
BPHYT_RS35385: BPHYT_RS35385 - GntR family transcriptional regulator, at 3,538,535 to 3,539,272
_RS35385
BPHYT_RS35390: BPHYT_RS35390 - MFS transporter, at 3,539,423 to 3,540,727
_RS35390
BPHYT_RS35395: BPHYT_RS35395 - membrane protein, at 3,540,742 to 3,541,611
_RS35395
BPHYT_RS35400: BPHYT_RS35400 - ABC transporter, at 3,541,616 to 3,543,280
_RS35400
Position (kb)
3539
3540
3541 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3538.759 kb on - strand, within BPHYT_RS35385 at 3538.827 kb on - strand, within BPHYT_RS35385 at 3539.133 kb on + strand, within BPHYT_RS35385 at 3539.134 kb on - strand, within BPHYT_RS35385 at 3539.288 kb on - strand at 3539.288 kb on - strand at 3539.304 kb on - strand at 3539.312 kb on + strand at 3539.313 kb on - strand at 3539.436 kb on - strand at 3539.674 kb on + strand, within BPHYT_RS35390 at 3539.674 kb on + strand, within BPHYT_RS35390 at 3539.895 kb on - strand, within BPHYT_RS35390 at 3540.069 kb on + strand, within BPHYT_RS35390 at 3540.069 kb on + strand, within BPHYT_RS35390 at 3540.141 kb on - strand, within BPHYT_RS35390 at 3540.338 kb on + strand, within BPHYT_RS35390 at 3540.339 kb on - strand, within BPHYT_RS35390 at 3540.934 kb on + strand, within BPHYT_RS35395 at 3540.934 kb on + strand, within BPHYT_RS35395 at 3540.935 kb on - strand, within BPHYT_RS35395 at 3541.327 kb on + strand, within BPHYT_RS35395 at 3541.617 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO6 MOI 1 remove 3,538,759 - BPHYT_RS35385 0.30 +0.6 3,538,827 - BPHYT_RS35385 0.40 -0.7 3,539,133 + BPHYT_RS35385 0.81 +1.8 3,539,134 - BPHYT_RS35385 0.81 -0.7 3,539,288 - -0.6 3,539,288 - -0.4 3,539,304 - -0.1 3,539,312 + +0.8 3,539,313 - +0.4 3,539,436 - -0.4 3,539,674 + BPHYT_RS35390 0.19 -0.5 3,539,674 + BPHYT_RS35390 0.19 +0.2 3,539,895 - BPHYT_RS35390 0.36 +0.9 3,540,069 + BPHYT_RS35390 0.50 +0.4 3,540,069 + BPHYT_RS35390 0.50 +0.3 3,540,141 - BPHYT_RS35390 0.55 +0.1 3,540,338 + BPHYT_RS35390 0.70 +0.2 3,540,339 - BPHYT_RS35390 0.70 -3.3 3,540,934 + BPHYT_RS35395 0.22 +1.8 3,540,934 + BPHYT_RS35395 0.22 -0.0 3,540,935 - BPHYT_RS35395 0.22 -1.2 3,541,327 + BPHYT_RS35395 0.67 -1.4 3,541,617 + +0.5
Or see this region's nucleotide sequence