Experiment: 24hr PHYTO6 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS35265 and BPHYT_RS35270 are separated by 9 nucleotides BPHYT_RS35270 and BPHYT_RS35275 are separated by 194 nucleotides BPHYT_RS35275 and BPHYT_RS35280 are separated by 78 nucleotides BPHYT_RS35280 and BPHYT_RS35285 are separated by 132 nucleotides
BPHYT_RS35265: BPHYT_RS35265 - LysR family transcriptional regulator, at 3,513,507 to 3,514,373
_RS35265
BPHYT_RS35270: BPHYT_RS35270 - hypothetical protein, at 3,514,383 to 3,515,057
_RS35270
BPHYT_RS35275: BPHYT_RS35275 - acetyltransferase, at 3,515,252 to 3,515,785
_RS35275
BPHYT_RS35280: BPHYT_RS35280 - acetyltransferase, at 3,515,864 to 3,516,601
_RS35280
BPHYT_RS35285: BPHYT_RS35285 - DNA-binding protein, at 3,516,734 to 3,518,257
_RS35285
Position (kb)
3515
3516 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3514.384 kb on + strand at 3514.384 kb on + strand at 3514.419 kb on + strand at 3514.507 kb on + strand, within BPHYT_RS35270 at 3514.507 kb on + strand, within BPHYT_RS35270 at 3514.578 kb on + strand, within BPHYT_RS35270 at 3514.579 kb on - strand, within BPHYT_RS35270 at 3514.579 kb on - strand, within BPHYT_RS35270 at 3514.631 kb on - strand, within BPHYT_RS35270 at 3514.720 kb on + strand, within BPHYT_RS35270 at 3515.138 kb on - strand at 3515.236 kb on + strand at 3515.237 kb on - strand at 3515.444 kb on + strand, within BPHYT_RS35275 at 3515.445 kb on - strand, within BPHYT_RS35275 at 3515.735 kb on + strand at 3515.736 kb on - strand at 3515.968 kb on + strand, within BPHYT_RS35280 at 3515.971 kb on - strand, within BPHYT_RS35280 at 3516.229 kb on - strand, within BPHYT_RS35280 at 3516.411 kb on + strand, within BPHYT_RS35280 at 3516.411 kb on + strand, within BPHYT_RS35280 at 3516.412 kb on - strand, within BPHYT_RS35280 at 3516.412 kb on - strand, within BPHYT_RS35280 at 3516.444 kb on + strand, within BPHYT_RS35280 at 3516.453 kb on + strand, within BPHYT_RS35280 at 3516.456 kb on - strand, within BPHYT_RS35280 at 3516.456 kb on - strand, within BPHYT_RS35280 at 3516.456 kb on - strand, within BPHYT_RS35280 at 3516.602 kb on - strand at 3516.643 kb on + strand at 3516.674 kb on - strand at 3516.693 kb on - strand at 3516.693 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO6 MOI 1 remove 3,514,384 + -0.3 3,514,384 + -0.8 3,514,419 + -0.4 3,514,507 + BPHYT_RS35270 0.18 -0.4 3,514,507 + BPHYT_RS35270 0.18 -2.1 3,514,578 + BPHYT_RS35270 0.29 -0.3 3,514,579 - BPHYT_RS35270 0.29 -3.3 3,514,579 - BPHYT_RS35270 0.29 +0.8 3,514,631 - BPHYT_RS35270 0.37 -1.2 3,514,720 + BPHYT_RS35270 0.50 +0.2 3,515,138 - -0.1 3,515,236 + +0.3 3,515,237 - -1.1 3,515,444 + BPHYT_RS35275 0.36 -2.4 3,515,445 - BPHYT_RS35275 0.36 -0.1 3,515,735 + +1.5 3,515,736 - -1.2 3,515,968 + BPHYT_RS35280 0.14 -0.9 3,515,971 - BPHYT_RS35280 0.14 +2.3 3,516,229 - BPHYT_RS35280 0.49 -0.3 3,516,411 + BPHYT_RS35280 0.74 -1.0 3,516,411 + BPHYT_RS35280 0.74 -0.1 3,516,412 - BPHYT_RS35280 0.74 +0.1 3,516,412 - BPHYT_RS35280 0.74 +2.3 3,516,444 + BPHYT_RS35280 0.79 -0.1 3,516,453 + BPHYT_RS35280 0.80 -0.9 3,516,456 - BPHYT_RS35280 0.80 -0.6 3,516,456 - BPHYT_RS35280 0.80 +0.5 3,516,456 - BPHYT_RS35280 0.80 -0.3 3,516,602 - +0.1 3,516,643 + -0.4 3,516,674 - -2.2 3,516,693 - -0.2 3,516,693 - +0.5
Or see this region's nucleotide sequence