Experiment: 24hr PHYTO6 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS32420 and BPHYT_RS32425 are separated by 35 nucleotides BPHYT_RS32425 and BPHYT_RS32430 are separated by 111 nucleotides BPHYT_RS32430 and BPHYT_RS32435 overlap by 14 nucleotides
BPHYT_RS32420: BPHYT_RS32420 - selenocysteine synthase, at 2,846,390 to 2,847,820
_RS32420
BPHYT_RS32425: BPHYT_RS32425 - formate dehydrogenase accessory protein FdhE, at 2,847,856 to 2,848,851
_RS32425
BPHYT_RS32430: BPHYT_RS32430 - formate dehydrogenase-N subunit gamma, at 2,848,963 to 2,849,619
_RS32430
BPHYT_RS32435: BPHYT_RS32435 - formate dehydrogenase-N subunit beta, at 2,849,606 to 2,850,538
_RS32435
Position (kb)
2847
2848
2849 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2847.114 kb on + strand, within BPHYT_RS32420 at 2847.114 kb on + strand, within BPHYT_RS32420 at 2847.115 kb on - strand, within BPHYT_RS32420 at 2847.171 kb on + strand, within BPHYT_RS32420 at 2847.610 kb on + strand, within BPHYT_RS32420 at 2847.718 kb on - strand at 2847.809 kb on + strand at 2848.035 kb on - strand, within BPHYT_RS32425 at 2848.223 kb on + strand, within BPHYT_RS32425 at 2848.223 kb on + strand, within BPHYT_RS32425 at 2848.224 kb on - strand, within BPHYT_RS32425 at 2848.224 kb on - strand, within BPHYT_RS32425 at 2848.352 kb on - strand, within BPHYT_RS32425 at 2848.517 kb on - strand, within BPHYT_RS32425 at 2848.517 kb on - strand, within BPHYT_RS32425 at 2848.854 kb on + strand at 2848.951 kb on - strand at 2848.951 kb on - strand at 2849.018 kb on + strand at 2849.018 kb on + strand at 2849.076 kb on - strand, within BPHYT_RS32430 at 2849.266 kb on - strand, within BPHYT_RS32430 at 2849.348 kb on + strand, within BPHYT_RS32430 at 2849.348 kb on + strand, within BPHYT_RS32430 at 2849.348 kb on + strand, within BPHYT_RS32430 at 2849.349 kb on - strand, within BPHYT_RS32430 at 2849.349 kb on - strand, within BPHYT_RS32430 at 2849.349 kb on - strand, within BPHYT_RS32430 at 2849.470 kb on - strand, within BPHYT_RS32430 at 2849.542 kb on - strand, within BPHYT_RS32430 at 2849.542 kb on - strand, within BPHYT_RS32430 at 2849.642 kb on + strand at 2849.713 kb on - strand, within BPHYT_RS32435
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO6 MOI 1 remove 2,847,114 + BPHYT_RS32420 0.51 -0.2 2,847,114 + BPHYT_RS32420 0.51 +0.8 2,847,115 - BPHYT_RS32420 0.51 -2.9 2,847,171 + BPHYT_RS32420 0.55 +0.4 2,847,610 + BPHYT_RS32420 0.85 -0.1 2,847,718 - +0.5 2,847,809 + +1.5 2,848,035 - BPHYT_RS32425 0.18 -0.8 2,848,223 + BPHYT_RS32425 0.37 -0.9 2,848,223 + BPHYT_RS32425 0.37 +0.3 2,848,224 - BPHYT_RS32425 0.37 +2.3 2,848,224 - BPHYT_RS32425 0.37 -1.3 2,848,352 - BPHYT_RS32425 0.50 +0.7 2,848,517 - BPHYT_RS32425 0.66 -0.7 2,848,517 - BPHYT_RS32425 0.66 -0.6 2,848,854 + +0.2 2,848,951 - -0.2 2,848,951 - +0.2 2,849,018 + +0.5 2,849,018 + +1.3 2,849,076 - BPHYT_RS32430 0.17 -1.0 2,849,266 - BPHYT_RS32430 0.46 -0.5 2,849,348 + BPHYT_RS32430 0.59 -1.4 2,849,348 + BPHYT_RS32430 0.59 +2.3 2,849,348 + BPHYT_RS32430 0.59 -1.0 2,849,349 - BPHYT_RS32430 0.59 -0.5 2,849,349 - BPHYT_RS32430 0.59 -1.0 2,849,349 - BPHYT_RS32430 0.59 -0.3 2,849,470 - BPHYT_RS32430 0.77 -2.0 2,849,542 - BPHYT_RS32430 0.88 +1.9 2,849,542 - BPHYT_RS32430 0.88 +0.7 2,849,642 + +0.4 2,849,713 - BPHYT_RS32435 0.11 +0.7
Or see this region's nucleotide sequence