Experiment: 24hr PHYTO6 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS32330 and BPHYT_RS32335 are separated by 333 nucleotides BPHYT_RS32335 and BPHYT_RS32340 are separated by 133 nucleotides BPHYT_RS32340 and BPHYT_RS32345 overlap by 4 nucleotides BPHYT_RS32345 and BPHYT_RS32350 are separated by 249 nucleotides
BPHYT_RS32330: BPHYT_RS32330 - 5-oxoprolinase, at 2,825,086 to 2,827,122
_RS32330
BPHYT_RS32335: BPHYT_RS32335 - AsnC family transcriptional regulator, at 2,827,456 to 2,827,881
_RS32335
BPHYT_RS32340: BPHYT_RS32340 - LrgA family protein, at 2,828,015 to 2,828,419
_RS32340
BPHYT_RS32345: BPHYT_RS32345 - LrgB family protein, at 2,828,416 to 2,829,096
_RS32345
BPHYT_RS32350: BPHYT_RS32350 - ABC transporter permease, at 2,829,346 to 2,830,158
_RS32350
Position (kb)
2828
2829 Strain fitness (log2 ratio)
-1
0
1 at 2827.105 kb on + strand at 2827.544 kb on - strand, within BPHYT_RS32335 at 2827.580 kb on - strand, within BPHYT_RS32335 at 2828.037 kb on + strand at 2828.109 kb on + strand, within BPHYT_RS32340 at 2828.110 kb on - strand, within BPHYT_RS32340 at 2828.237 kb on - strand, within BPHYT_RS32340 at 2828.351 kb on - strand, within BPHYT_RS32340 at 2828.479 kb on + strand at 2828.480 kb on - strand at 2828.480 kb on - strand at 2828.567 kb on - strand, within BPHYT_RS32345 at 2828.576 kb on - strand, within BPHYT_RS32345 at 2828.576 kb on - strand, within BPHYT_RS32345 at 2828.661 kb on + strand, within BPHYT_RS32345 at 2828.887 kb on - strand, within BPHYT_RS32345 at 2829.102 kb on - strand at 2829.288 kb on - strand at 2829.328 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO6 MOI 1 remove 2,827,105 + -0.1 2,827,544 - BPHYT_RS32335 0.21 +1.0 2,827,580 - BPHYT_RS32335 0.29 -0.2 2,828,037 + +0.0 2,828,109 + BPHYT_RS32340 0.23 -1.1 2,828,110 - BPHYT_RS32340 0.23 +0.1 2,828,237 - BPHYT_RS32340 0.55 -1.4 2,828,351 - BPHYT_RS32340 0.83 -1.0 2,828,479 + +0.2 2,828,480 - +0.1 2,828,480 - +0.6 2,828,567 - BPHYT_RS32345 0.22 -0.3 2,828,576 - BPHYT_RS32345 0.23 -0.3 2,828,576 - BPHYT_RS32345 0.23 +0.5 2,828,661 + BPHYT_RS32345 0.36 -0.4 2,828,887 - BPHYT_RS32345 0.69 +0.3 2,829,102 - -0.3 2,829,288 - -0.0 2,829,328 + -1.5
Or see this region's nucleotide sequence