Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS29985

Experiment: 24hr PHYTO6 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS29975 and BPHYT_RS29980 are separated by 390 nucleotidesBPHYT_RS29980 and BPHYT_RS29985 are separated by 141 nucleotidesBPHYT_RS29985 and BPHYT_RS29990 overlap by 4 nucleotides BPHYT_RS29975: BPHYT_RS29975 - multidrug ABC transporter ATP-binding protein, at 2,279,053 to 2,279,979 _RS29975 BPHYT_RS29980: BPHYT_RS29980 - hypothetical protein, at 2,280,370 to 2,280,792 _RS29980 BPHYT_RS29985: BPHYT_RS29985 - feruloyl esterase, at 2,280,934 to 2,282,604 _RS29985 BPHYT_RS29990: BPHYT_RS29990 - hypothetical protein, at 2,282,601 to 2,283,734 _RS29990 Position (kb) 2280 2281 2282 2283Strain fitness (log2 ratio) -1 0 1at 2280.051 kb on + strandat 2280.313 kb on + strandat 2280.314 kb on - strandat 2280.957 kb on + strandat 2280.958 kb on - strandat 2281.230 kb on - strand, within BPHYT_RS29985at 2281.440 kb on + strand, within BPHYT_RS29985at 2281.595 kb on + strand, within BPHYT_RS29985at 2281.991 kb on + strand, within BPHYT_RS29985at 2282.230 kb on - strand, within BPHYT_RS29985at 2282.745 kb on - strand, within BPHYT_RS29990at 2283.091 kb on - strand, within BPHYT_RS29990at 2283.154 kb on - strand, within BPHYT_RS29990at 2283.527 kb on - strand, within BPHYT_RS29990

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Per-strain Table

Position Strand Gene LocusTag Fraction 24hr PHYTO6 MOI 1
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2,280,051 + -0.6
2,280,313 + -0.2
2,280,314 - +0.3
2,280,957 + -1.2
2,280,958 - -0.3
2,281,230 - BPHYT_RS29985 0.18 +0.6
2,281,440 + BPHYT_RS29985 0.30 -0.2
2,281,595 + BPHYT_RS29985 0.40 -0.9
2,281,991 + BPHYT_RS29985 0.63 +0.8
2,282,230 - BPHYT_RS29985 0.78 +0.9
2,282,745 - BPHYT_RS29990 0.13 +0.1
2,283,091 - BPHYT_RS29990 0.43 -1.0
2,283,154 - BPHYT_RS29990 0.49 -1.5
2,283,527 - BPHYT_RS29990 0.82 -0.4

Or see this region's nucleotide sequence