Experiment: 24hr PHYTO6 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS29770 and BPHYT_RS29775 are separated by 273 nucleotides BPHYT_RS29775 and BPHYT_RS29780 are separated by 39 nucleotides BPHYT_RS29780 and BPHYT_RS29785 are separated by 308 nucleotides BPHYT_RS29785 and BPHYT_RS29790 are separated by 51 nucleotides
BPHYT_RS29770: BPHYT_RS29770 - diguanylate cyclase, at 2,234,036 to 2,235,151
_RS29770
BPHYT_RS29775: BPHYT_RS29775 - hypothetical protein, at 2,235,425 to 2,235,682
_RS29775
BPHYT_RS29780: BPHYT_RS29780 - DSBA oxidoreductase, at 2,235,722 to 2,236,360
_RS29780
BPHYT_RS29785: BPHYT_RS29785 - hypothetical protein, at 2,236,669 to 2,237,037
_RS29785
BPHYT_RS29790: BPHYT_RS29790 - lysine transporter LysE, at 2,237,089 to 2,237,715
_RS29790
Position (kb)
2235
2236
2237 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2235.205 kb on - strand at 2235.207 kb on + strand at 2235.252 kb on - strand at 2235.284 kb on - strand at 2235.621 kb on + strand, within BPHYT_RS29775 at 2235.680 kb on + strand at 2235.681 kb on - strand at 2235.750 kb on + strand at 2236.005 kb on + strand, within BPHYT_RS29780 at 2236.104 kb on + strand, within BPHYT_RS29780 at 2236.105 kb on - strand, within BPHYT_RS29780 at 2236.105 kb on - strand, within BPHYT_RS29780 at 2236.140 kb on + strand, within BPHYT_RS29780 at 2236.140 kb on + strand, within BPHYT_RS29780 at 2236.141 kb on - strand, within BPHYT_RS29780 at 2236.282 kb on - strand, within BPHYT_RS29780 at 2236.287 kb on + strand, within BPHYT_RS29780 at 2236.288 kb on - strand, within BPHYT_RS29780 at 2236.397 kb on - strand at 2236.397 kb on - strand at 2236.433 kb on - strand at 2236.459 kb on + strand at 2236.475 kb on - strand at 2236.475 kb on - strand at 2236.484 kb on + strand at 2236.485 kb on - strand at 2236.568 kb on + strand at 2236.635 kb on - strand at 2237.035 kb on + strand at 2237.226 kb on + strand, within BPHYT_RS29790 at 2237.229 kb on - strand, within BPHYT_RS29790 at 2237.353 kb on + strand, within BPHYT_RS29790
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO6 MOI 1 remove 2,235,205 - -0.5 2,235,207 + +0.4 2,235,252 - +0.3 2,235,284 - +0.2 2,235,621 + BPHYT_RS29775 0.76 +1.0 2,235,680 + -1.6 2,235,681 - -0.2 2,235,750 + -1.1 2,236,005 + BPHYT_RS29780 0.44 +0.9 2,236,104 + BPHYT_RS29780 0.60 -2.8 2,236,105 - BPHYT_RS29780 0.60 +0.1 2,236,105 - BPHYT_RS29780 0.60 +0.3 2,236,140 + BPHYT_RS29780 0.65 +0.3 2,236,140 + BPHYT_RS29780 0.65 -2.0 2,236,141 - BPHYT_RS29780 0.66 -0.8 2,236,282 - BPHYT_RS29780 0.88 +0.1 2,236,287 + BPHYT_RS29780 0.88 +0.2 2,236,288 - BPHYT_RS29780 0.89 +0.4 2,236,397 - -0.4 2,236,397 - -0.4 2,236,433 - +0.7 2,236,459 + +2.4 2,236,475 - -0.2 2,236,475 - +0.7 2,236,484 + -0.4 2,236,485 - +0.4 2,236,568 + -0.4 2,236,635 - -1.0 2,237,035 + -0.8 2,237,226 + BPHYT_RS29790 0.22 +0.4 2,237,229 - BPHYT_RS29790 0.22 -1.0 2,237,353 + BPHYT_RS29790 0.42 -0.4
Or see this region's nucleotide sequence