Experiment: 24hr PHYTO6 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS23215 and BPHYT_RS23220 are separated by 234 nucleotides BPHYT_RS23220 and BPHYT_RS23225 are separated by 113 nucleotides BPHYT_RS23225 and BPHYT_RS23230 are separated by 30 nucleotides
BPHYT_RS23215: BPHYT_RS23215 - transcriptional regulator, at 773,475 to 774,413
_RS23215
BPHYT_RS23220: BPHYT_RS23220 - hypothetical protein, at 774,648 to 775,379
_RS23220
BPHYT_RS23225: BPHYT_RS23225 - hypothetical protein, at 775,493 to 775,699
_RS23225
BPHYT_RS23230: BPHYT_RS23230 - histidine kinase, at 775,730 to 777,703
_RS23230
Position (kb) 774
775
776 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 774.199 kb on + strand, within BPHYT_RS23215 at 774.199 kb on + strand, within BPHYT_RS23215 at 774.199 kb on + strand, within BPHYT_RS23215 at 774.209 kb on - strand, within BPHYT_RS23215 at 774.659 kb on - strand at 774.659 kb on - strand at 774.741 kb on + strand, within BPHYT_RS23220 at 775.111 kb on - strand, within BPHYT_RS23220 at 775.313 kb on + strand at 775.608 kb on - strand, within BPHYT_RS23225 at 775.610 kb on + strand, within BPHYT_RS23225 at 775.752 kb on + strand at 776.257 kb on - strand, within BPHYT_RS23230
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO6 MOI 1 remove 774,199 + BPHYT_RS23215 0.77 +1.4 774,199 + BPHYT_RS23215 0.77 -0.0 774,199 + BPHYT_RS23215 0.77 +0.1 774,209 - BPHYT_RS23215 0.78 -1.5 774,659 - +0.1 774,659 - +0.6 774,741 + BPHYT_RS23220 0.13 +0.1 775,111 - BPHYT_RS23220 0.63 +0.7 775,313 + -0.4 775,608 - BPHYT_RS23225 0.56 -2.8 775,610 + BPHYT_RS23225 0.57 -2.0 775,752 + +0.7 776,257 - BPHYT_RS23230 0.27 +0.4
Or see this region's nucleotide sequence