Experiment: 24hr PHYTO6 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS21315 and BPHYT_RS21320 are separated by 216 nucleotides BPHYT_RS21320 and BPHYT_RS21325 are separated by 229 nucleotides BPHYT_RS21325 and BPHYT_RS21330 are separated by 384 nucleotides
BPHYT_RS21315: BPHYT_RS21315 - hypothetical protein, at 341,401 to 341,937
_RS21315
BPHYT_RS21320: BPHYT_RS21320 - alkaline phosphatase, at 342,154 to 344,106
_RS21320
BPHYT_RS21325: BPHYT_RS21325 - hypothetical protein, at 344,336 to 344,674
_RS21325
BPHYT_RS21330: BPHYT_RS21330 - hypothetical protein, at 345,059 to 345,493
_RS21330
Position (kb) 342
343
344
345 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 341.245 kb on + strand at 341.245 kb on + strand at 341.245 kb on + strand at 341.727 kb on + strand, within BPHYT_RS21315 at 341.809 kb on + strand, within BPHYT_RS21315 at 341.833 kb on - strand, within BPHYT_RS21315 at 341.833 kb on - strand, within BPHYT_RS21315 at 341.872 kb on - strand, within BPHYT_RS21315 at 341.872 kb on - strand, within BPHYT_RS21315 at 342.010 kb on + strand at 342.010 kb on + strand at 342.014 kb on - strand at 342.014 kb on - strand at 342.026 kb on + strand at 342.028 kb on + strand at 342.029 kb on - strand at 342.048 kb on - strand at 342.048 kb on - strand at 342.439 kb on + strand, within BPHYT_RS21320 at 342.440 kb on - strand, within BPHYT_RS21320 at 342.571 kb on + strand, within BPHYT_RS21320 at 342.703 kb on + strand, within BPHYT_RS21320 at 342.703 kb on + strand, within BPHYT_RS21320 at 342.704 kb on - strand, within BPHYT_RS21320 at 342.781 kb on + strand, within BPHYT_RS21320 at 342.796 kb on - strand, within BPHYT_RS21320 at 343.031 kb on - strand, within BPHYT_RS21320 at 343.171 kb on + strand, within BPHYT_RS21320 at 343.171 kb on + strand, within BPHYT_RS21320 at 343.172 kb on - strand, within BPHYT_RS21320 at 343.336 kb on + strand, within BPHYT_RS21320 at 343.351 kb on + strand, within BPHYT_RS21320 at 343.351 kb on + strand, within BPHYT_RS21320 at 343.352 kb on - strand, within BPHYT_RS21320 at 343.352 kb on - strand, within BPHYT_RS21320 at 343.426 kb on + strand, within BPHYT_RS21320 at 343.485 kb on + strand, within BPHYT_RS21320 at 343.485 kb on + strand, within BPHYT_RS21320 at 343.502 kb on + strand, within BPHYT_RS21320 at 343.502 kb on + strand, within BPHYT_RS21320 at 343.502 kb on + strand, within BPHYT_RS21320 at 343.502 kb on + strand, within BPHYT_RS21320 at 343.503 kb on - strand, within BPHYT_RS21320 at 343.503 kb on - strand, within BPHYT_RS21320 at 343.503 kb on - strand, within BPHYT_RS21320 at 343.601 kb on - strand, within BPHYT_RS21320 at 343.601 kb on - strand, within BPHYT_RS21320 at 343.849 kb on + strand, within BPHYT_RS21320 at 343.948 kb on + strand at 344.042 kb on - strand at 344.042 kb on - strand at 344.042 kb on - strand at 344.042 kb on - strand at 344.104 kb on + strand at 344.104 kb on + strand at 344.234 kb on + strand at 344.234 kb on + strand at 344.234 kb on + strand at 344.234 kb on + strand at 344.234 kb on + strand at 344.234 kb on + strand at 344.235 kb on - strand at 344.237 kb on - strand at 344.237 kb on - strand at 344.259 kb on - strand at 344.314 kb on + strand at 344.353 kb on + strand at 344.474 kb on + strand, within BPHYT_RS21325 at 344.531 kb on + strand, within BPHYT_RS21325 at 344.531 kb on + strand, within BPHYT_RS21325 at 344.663 kb on + strand at 344.748 kb on + strand at 344.812 kb on + strand at 344.812 kb on + strand at 344.868 kb on - strand at 344.943 kb on + strand at 344.943 kb on + strand at 344.944 kb on - strand at 344.984 kb on + strand at 344.984 kb on + strand at 344.984 kb on + strand at 344.985 kb on - strand at 344.987 kb on - strand at 344.994 kb on - strand at 345.009 kb on + strand at 345.009 kb on + strand at 345.009 kb on + strand at 345.009 kb on + strand at 345.010 kb on - strand at 345.010 kb on - strand at 345.086 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO6 MOI 1 remove 341,245 + -0.5 341,245 + +0.2 341,245 + -0.3 341,727 + BPHYT_RS21315 0.61 +0.4 341,809 + BPHYT_RS21315 0.76 -0.9 341,833 - BPHYT_RS21315 0.80 -2.1 341,833 - BPHYT_RS21315 0.80 -0.4 341,872 - BPHYT_RS21315 0.88 -1.8 341,872 - BPHYT_RS21315 0.88 +0.3 342,010 + +1.3 342,010 + +0.4 342,014 - +0.7 342,014 - +0.7 342,026 + -0.2 342,028 + +0.5 342,029 - -0.0 342,048 - -0.7 342,048 - -0.9 342,439 + BPHYT_RS21320 0.15 -0.5 342,440 - BPHYT_RS21320 0.15 -1.7 342,571 + BPHYT_RS21320 0.21 -1.1 342,703 + BPHYT_RS21320 0.28 +0.4 342,703 + BPHYT_RS21320 0.28 -1.0 342,704 - BPHYT_RS21320 0.28 -1.0 342,781 + BPHYT_RS21320 0.32 +0.3 342,796 - BPHYT_RS21320 0.33 +0.9 343,031 - BPHYT_RS21320 0.45 +0.6 343,171 + BPHYT_RS21320 0.52 +0.1 343,171 + BPHYT_RS21320 0.52 -0.3 343,172 - BPHYT_RS21320 0.52 -0.2 343,336 + BPHYT_RS21320 0.61 +0.1 343,351 + BPHYT_RS21320 0.61 +0.6 343,351 + BPHYT_RS21320 0.61 -0.0 343,352 - BPHYT_RS21320 0.61 -0.4 343,352 - BPHYT_RS21320 0.61 +2.3 343,426 + BPHYT_RS21320 0.65 +0.6 343,485 + BPHYT_RS21320 0.68 -0.8 343,485 + BPHYT_RS21320 0.68 -0.8 343,502 + BPHYT_RS21320 0.69 +0.3 343,502 + BPHYT_RS21320 0.69 -0.6 343,502 + BPHYT_RS21320 0.69 +0.8 343,502 + BPHYT_RS21320 0.69 -0.5 343,503 - BPHYT_RS21320 0.69 -0.8 343,503 - BPHYT_RS21320 0.69 -0.3 343,503 - BPHYT_RS21320 0.69 -1.2 343,601 - BPHYT_RS21320 0.74 +0.1 343,601 - BPHYT_RS21320 0.74 +0.7 343,849 + BPHYT_RS21320 0.87 -0.3 343,948 + -2.9 344,042 - -0.3 344,042 - +1.1 344,042 - -2.6 344,042 - -2.6 344,104 + -0.9 344,104 + +0.5 344,234 + -0.5 344,234 + +0.8 344,234 + +0.2 344,234 + +0.8 344,234 + +2.3 344,234 + +0.3 344,235 - -1.1 344,237 - -0.8 344,237 - +1.6 344,259 - -2.6 344,314 + +0.2 344,353 + -2.2 344,474 + BPHYT_RS21325 0.41 -2.2 344,531 + BPHYT_RS21325 0.58 -1.5 344,531 + BPHYT_RS21325 0.58 -1.2 344,663 + -0.2 344,748 + +0.1 344,812 + -0.4 344,812 + +0.3 344,868 - -1.1 344,943 + -0.1 344,943 + +0.4 344,944 - -0.1 344,984 + -0.0 344,984 + -0.8 344,984 + -1.3 344,985 - +0.3 344,987 - -1.8 344,994 - -0.2 345,009 + +0.5 345,009 + +0.7 345,009 + +0.3 345,009 + -0.4 345,010 - -0.4 345,010 - -0.4 345,086 - -0.3
Or see this region's nucleotide sequence