Experiment: 24hr PHYTO6 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS20105 and BPHYT_RS20110 are separated by 66 nucleotides BPHYT_RS20110 and BPHYT_RS20115 are separated by 25 nucleotides BPHYT_RS20115 and BPHYT_RS20120 are separated by 182 nucleotides
BPHYT_RS20105: BPHYT_RS20105 - nitrate ABC transporter substrate-binding protein, at 53,379 to 54,419
_RS20105
BPHYT_RS20110: BPHYT_RS20110 - sensor histidine kinase, at 54,486 to 55,868
_RS20110
BPHYT_RS20115: BPHYT_RS20115 - transcriptional regulator, at 55,894 to 56,592
_RS20115
BPHYT_RS20120: BPHYT_RS20120 - porin, at 56,775 to 57,899
_RS20120
Position (kb) 54
55
56 Strain fitness (log2 ratio) -2
-1
0
1
2 at 53.895 kb on + strand, within BPHYT_RS20105 at 53.896 kb on - strand, within BPHYT_RS20105 at 54.035 kb on + strand, within BPHYT_RS20105 at 54.036 kb on - strand, within BPHYT_RS20105 at 54.421 kb on - strand at 54.738 kb on + strand, within BPHYT_RS20110 at 54.979 kb on + strand, within BPHYT_RS20110 at 55.023 kb on + strand, within BPHYT_RS20110 at 55.628 kb on + strand, within BPHYT_RS20110 at 55.877 kb on + strand at 56.026 kb on + strand, within BPHYT_RS20115 at 56.469 kb on + strand, within BPHYT_RS20115 at 56.475 kb on + strand, within BPHYT_RS20115 at 56.625 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO6 MOI 1 remove 53,895 + BPHYT_RS20105 0.50 -0.3 53,896 - BPHYT_RS20105 0.50 +0.7 54,035 + BPHYT_RS20105 0.63 -0.9 54,036 - BPHYT_RS20105 0.63 -1.4 54,421 - -0.5 54,738 + BPHYT_RS20110 0.18 +1.3 54,979 + BPHYT_RS20110 0.36 -0.3 55,023 + BPHYT_RS20110 0.39 +0.1 55,628 + BPHYT_RS20110 0.83 -1.6 55,877 + -1.1 56,026 + BPHYT_RS20115 0.19 +2.0 56,469 + BPHYT_RS20115 0.82 +0.8 56,475 + BPHYT_RS20115 0.83 +1.1 56,625 - -2.6
Or see this region's nucleotide sequence