Experiment: 24hr PHYTO6 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS17405 and BPHYT_RS17410 are separated by 46 nucleotides BPHYT_RS17410 and BPHYT_RS17415 are separated by 84 nucleotides BPHYT_RS17415 and BPHYT_RS17420 are separated by 128 nucleotides BPHYT_RS17420 and BPHYT_RS17425 are separated by 154 nucleotides
BPHYT_RS17405: BPHYT_RS17405 - indole-3-glycerol phosphate synthase, at 3,943,673 to 3,944,458
_RS17405
BPHYT_RS17410: BPHYT_RS17410 - adenylate cyclase, at 3,944,505 to 3,945,131
_RS17410
BPHYT_RS17415: BPHYT_RS17415 - uracil-DNA glycosylase, at 3,945,216 to 3,946,061
_RS17415
BPHYT_RS17420: BPHYT_RS17420 - FMN-dependent NADH-azoreductase, at 3,946,190 to 3,946,786
_RS17420
BPHYT_RS17425: BPHYT_RS17425 - peptidase M61, at 3,946,941 to 3,948,731
_RS17425
Position (kb)
3945
3946
3947 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3944.492 kb on - strand at 3944.492 kb on - strand at 3944.618 kb on + strand, within BPHYT_RS17410 at 3944.622 kb on + strand, within BPHYT_RS17410 at 3944.623 kb on - strand, within BPHYT_RS17410 at 3945.067 kb on + strand, within BPHYT_RS17410 at 3945.067 kb on + strand, within BPHYT_RS17410 at 3945.112 kb on - strand at 3946.059 kb on + strand at 3946.059 kb on + strand at 3946.191 kb on + strand at 3946.192 kb on - strand at 3946.269 kb on + strand, within BPHYT_RS17420 at 3946.380 kb on + strand, within BPHYT_RS17420 at 3946.381 kb on - strand, within BPHYT_RS17420 at 3946.381 kb on - strand, within BPHYT_RS17420 at 3946.381 kb on - strand, within BPHYT_RS17420 at 3946.436 kb on - strand, within BPHYT_RS17420 at 3946.464 kb on + strand, within BPHYT_RS17420 at 3946.464 kb on + strand, within BPHYT_RS17420 at 3946.464 kb on + strand, within BPHYT_RS17420 at 3946.465 kb on - strand, within BPHYT_RS17420 at 3946.497 kb on + strand, within BPHYT_RS17420 at 3946.891 kb on - strand at 3947.008 kb on + strand at 3947.008 kb on + strand at 3947.009 kb on - strand at 3947.009 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO6 MOI 1 remove 3,944,492 - -1.2 3,944,492 - -1.3 3,944,618 + BPHYT_RS17410 0.18 +0.3 3,944,622 + BPHYT_RS17410 0.19 +0.2 3,944,623 - BPHYT_RS17410 0.19 -0.2 3,945,067 + BPHYT_RS17410 0.90 -0.1 3,945,067 + BPHYT_RS17410 0.90 -0.3 3,945,112 - +0.3 3,946,059 + -1.5 3,946,059 + -1.1 3,946,191 + -0.0 3,946,192 - -0.5 3,946,269 + BPHYT_RS17420 0.13 +0.8 3,946,380 + BPHYT_RS17420 0.32 +0.4 3,946,381 - BPHYT_RS17420 0.32 -0.3 3,946,381 - BPHYT_RS17420 0.32 +0.8 3,946,381 - BPHYT_RS17420 0.32 +0.1 3,946,436 - BPHYT_RS17420 0.41 +2.8 3,946,464 + BPHYT_RS17420 0.46 -3.0 3,946,464 + BPHYT_RS17420 0.46 -0.2 3,946,464 + BPHYT_RS17420 0.46 -1.9 3,946,465 - BPHYT_RS17420 0.46 +0.2 3,946,497 + BPHYT_RS17420 0.51 +0.5 3,946,891 - +0.1 3,947,008 + -0.2 3,947,008 + +0.9 3,947,009 - -0.1 3,947,009 - +0.1
Or see this region's nucleotide sequence