Experiment: 24hr PHYTO6 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS16930 and BPHYT_RS16935 are separated by 133 nucleotides BPHYT_RS16935 and BPHYT_RS16940 are separated by 130 nucleotides BPHYT_RS16940 and BPHYT_RS16945 overlap by 4 nucleotides
BPHYT_RS16930: BPHYT_RS16930 - D-galactose ABC transporter, ATPase component (from data) , at 3,838,756 to 3,840,294
_RS16930
BPHYT_RS16935: BPHYT_RS16935 - D-galactose ABC transporter, substrate-binding component (from data) , at 3,840,428 to 3,841,426
_RS16935
BPHYT_RS16940: BPHYT_RS16940 - short-chain dehydrogenase, at 3,841,557 to 3,842,333
_RS16940
BPHYT_RS16945: BPHYT_RS16945 - 2-dehydro-3-deoxy-6-phosphogalactonate aldolase, at 3,842,330 to 3,843,010
_RS16945
Position (kb)
3840
3841
3842 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3839.556 kb on + strand, within BPHYT_RS16930 at 3839.557 kb on - strand, within BPHYT_RS16930 at 3839.558 kb on + strand, within BPHYT_RS16930 at 3839.558 kb on + strand, within BPHYT_RS16930 at 3839.558 kb on + strand, within BPHYT_RS16930 at 3839.558 kb on + strand, within BPHYT_RS16930 at 3839.558 kb on + strand, within BPHYT_RS16930 at 3839.558 kb on + strand, within BPHYT_RS16930 at 3839.558 kb on + strand, within BPHYT_RS16930 at 3839.559 kb on - strand, within BPHYT_RS16930 at 3839.559 kb on - strand, within BPHYT_RS16930 at 3839.559 kb on - strand, within BPHYT_RS16930 at 3839.561 kb on - strand, within BPHYT_RS16930 at 3839.563 kb on - strand, within BPHYT_RS16930 at 3839.684 kb on + strand, within BPHYT_RS16930 at 3839.711 kb on + strand, within BPHYT_RS16930 at 3839.711 kb on + strand, within BPHYT_RS16930 at 3839.711 kb on + strand, within BPHYT_RS16930 at 3839.711 kb on + strand, within BPHYT_RS16930 at 3839.711 kb on + strand, within BPHYT_RS16930 at 3839.712 kb on - strand, within BPHYT_RS16930 at 3839.712 kb on - strand, within BPHYT_RS16930 at 3840.135 kb on - strand, within BPHYT_RS16930 at 3840.267 kb on + strand at 3840.331 kb on + strand at 3840.345 kb on + strand at 3840.345 kb on + strand at 3840.346 kb on - strand at 3840.346 kb on - strand at 3840.412 kb on + strand at 3840.413 kb on - strand at 3840.543 kb on + strand, within BPHYT_RS16935 at 3840.543 kb on + strand, within BPHYT_RS16935 at 3840.543 kb on + strand, within BPHYT_RS16935 at 3840.543 kb on + strand, within BPHYT_RS16935 at 3840.544 kb on - strand, within BPHYT_RS16935 at 3840.744 kb on + strand, within BPHYT_RS16935 at 3840.924 kb on + strand, within BPHYT_RS16935 at 3840.925 kb on - strand, within BPHYT_RS16935 at 3841.017 kb on + strand, within BPHYT_RS16935 at 3841.017 kb on + strand, within BPHYT_RS16935 at 3841.027 kb on + strand, within BPHYT_RS16935 at 3841.033 kb on + strand, within BPHYT_RS16935 at 3841.444 kb on + strand at 3841.445 kb on - strand at 3841.445 kb on - strand at 3841.445 kb on - strand at 3841.520 kb on + strand at 3841.771 kb on + strand, within BPHYT_RS16940 at 3841.772 kb on - strand, within BPHYT_RS16940 at 3841.813 kb on + strand, within BPHYT_RS16940 at 3842.073 kb on + strand, within BPHYT_RS16940 at 3842.073 kb on + strand, within BPHYT_RS16940 at 3842.073 kb on + strand, within BPHYT_RS16940 at 3842.073 kb on + strand, within BPHYT_RS16940 at 3842.074 kb on - strand, within BPHYT_RS16940 at 3842.373 kb on + strand at 3842.374 kb on - strand at 3842.419 kb on + strand, within BPHYT_RS16945 at 3842.421 kb on + strand, within BPHYT_RS16945 at 3842.421 kb on + strand, within BPHYT_RS16945 at 3842.422 kb on - strand, within BPHYT_RS16945 at 3842.422 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO6 MOI 1 remove 3,839,556 + BPHYT_RS16930 0.52 -1.2 3,839,557 - BPHYT_RS16930 0.52 -1.2 3,839,558 + BPHYT_RS16930 0.52 +0.7 3,839,558 + BPHYT_RS16930 0.52 -0.1 3,839,558 + BPHYT_RS16930 0.52 -1.2 3,839,558 + BPHYT_RS16930 0.52 +1.2 3,839,558 + BPHYT_RS16930 0.52 -0.8 3,839,558 + BPHYT_RS16930 0.52 +0.1 3,839,558 + BPHYT_RS16930 0.52 -1.0 3,839,559 - BPHYT_RS16930 0.52 +0.0 3,839,559 - BPHYT_RS16930 0.52 -0.0 3,839,559 - BPHYT_RS16930 0.52 +1.9 3,839,561 - BPHYT_RS16930 0.52 +0.9 3,839,563 - BPHYT_RS16930 0.52 +0.1 3,839,684 + BPHYT_RS16930 0.60 -0.2 3,839,711 + BPHYT_RS16930 0.62 +1.0 3,839,711 + BPHYT_RS16930 0.62 -0.5 3,839,711 + BPHYT_RS16930 0.62 -0.9 3,839,711 + BPHYT_RS16930 0.62 -0.8 3,839,711 + BPHYT_RS16930 0.62 -2.7 3,839,712 - BPHYT_RS16930 0.62 -1.8 3,839,712 - BPHYT_RS16930 0.62 -1.5 3,840,135 - BPHYT_RS16930 0.90 -0.6 3,840,267 + +0.0 3,840,331 + -0.1 3,840,345 + -0.8 3,840,345 + -0.6 3,840,346 - -1.2 3,840,346 - -1.3 3,840,412 + -0.8 3,840,413 - -0.1 3,840,543 + BPHYT_RS16935 0.12 -2.3 3,840,543 + BPHYT_RS16935 0.12 +1.1 3,840,543 + BPHYT_RS16935 0.12 -0.6 3,840,543 + BPHYT_RS16935 0.12 -1.7 3,840,544 - BPHYT_RS16935 0.12 +0.7 3,840,744 + BPHYT_RS16935 0.32 -2.1 3,840,924 + BPHYT_RS16935 0.50 +0.3 3,840,925 - BPHYT_RS16935 0.50 -2.3 3,841,017 + BPHYT_RS16935 0.59 -1.0 3,841,017 + BPHYT_RS16935 0.59 -1.1 3,841,027 + BPHYT_RS16935 0.60 +0.3 3,841,033 + BPHYT_RS16935 0.61 -0.9 3,841,444 + -0.0 3,841,445 - +0.8 3,841,445 - -0.7 3,841,445 - -1.2 3,841,520 + +0.9 3,841,771 + BPHYT_RS16940 0.28 -2.2 3,841,772 - BPHYT_RS16940 0.28 +0.4 3,841,813 + BPHYT_RS16940 0.33 -1.9 3,842,073 + BPHYT_RS16940 0.66 -1.8 3,842,073 + BPHYT_RS16940 0.66 -0.2 3,842,073 + BPHYT_RS16940 0.66 -1.7 3,842,073 + BPHYT_RS16940 0.66 -1.5 3,842,074 - BPHYT_RS16940 0.67 -1.1 3,842,373 + +0.4 3,842,374 - -0.8 3,842,419 + BPHYT_RS16945 0.13 -0.3 3,842,421 + BPHYT_RS16945 0.13 -0.3 3,842,421 + BPHYT_RS16945 0.13 +0.0 3,842,422 - BPHYT_RS16945 0.14 +1.8 3,842,422 - +2.2
Or see this region's nucleotide sequence