Experiment: 24hr PHYTO6 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS13510 and BPHYT_RS13515 are separated by 100 nucleotides BPHYT_RS13515 and BPHYT_RS13520 overlap by 4 nucleotides BPHYT_RS13520 and BPHYT_RS13525 are separated by 17 nucleotides BPHYT_RS13525 and BPHYT_RS13530 are separated by 94 nucleotides
BPHYT_RS13510: BPHYT_RS13510 - C4-dicarboxylate transporter, at 3,082,498 to 3,083,745
_RS13510
BPHYT_RS13515: BPHYT_RS13515 - 3-isopropylmalate dehydratase small subunit, at 3,083,846 to 3,084,457
_RS13515
BPHYT_RS13520: BPHYT_RS13520 - isopropylmalate isomerase, at 3,084,454 to 3,085,869
_RS13520
BPHYT_RS13525: BPHYT_RS13525 - alpha/beta hydrolase, at 3,085,887 to 3,086,765
_RS13525
BPHYT_RS13530: BPHYT_RS13530 - LysR family transcriptional regulator, at 3,086,860 to 3,087,762
_RS13530
Position (kb)
3084
3085
3086 Strain fitness (log2 ratio)
-2
-1
0
1 at 3083.562 kb on + strand, within BPHYT_RS13510 at 3083.715 kb on - strand at 3083.715 kb on - strand at 3083.727 kb on - strand at 3083.847 kb on + strand at 3083.848 kb on - strand at 3084.267 kb on + strand, within BPHYT_RS13515 at 3084.268 kb on - strand, within BPHYT_RS13515 at 3084.331 kb on - strand at 3084.331 kb on - strand, within BPHYT_RS13515 at 3084.889 kb on - strand, within BPHYT_RS13520 at 3084.890 kb on + strand, within BPHYT_RS13520 at 3085.034 kb on + strand, within BPHYT_RS13520 at 3085.035 kb on - strand, within BPHYT_RS13520 at 3085.097 kb on + strand, within BPHYT_RS13520 at 3085.268 kb on + strand, within BPHYT_RS13520 at 3085.464 kb on - strand, within BPHYT_RS13520 at 3085.763 kb on + strand at 3085.764 kb on - strand at 3085.948 kb on + strand at 3085.948 kb on + strand at 3086.793 kb on + strand at 3086.794 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO6 MOI 1 remove 3,083,562 + BPHYT_RS13510 0.85 -0.3 3,083,715 - -0.3 3,083,715 - -0.0 3,083,727 - -0.7 3,083,847 + -0.2 3,083,848 - +1.1 3,084,267 + BPHYT_RS13515 0.69 -0.6 3,084,268 - BPHYT_RS13515 0.69 -0.5 3,084,331 - +1.6 3,084,331 - BPHYT_RS13515 0.79 -1.1 3,084,889 - BPHYT_RS13520 0.31 -1.2 3,084,890 + BPHYT_RS13520 0.31 +0.4 3,085,034 + BPHYT_RS13520 0.41 -0.6 3,085,035 - BPHYT_RS13520 0.41 +0.2 3,085,097 + BPHYT_RS13520 0.45 -1.4 3,085,268 + BPHYT_RS13520 0.57 -0.9 3,085,464 - BPHYT_RS13520 0.71 +0.6 3,085,763 + +0.8 3,085,764 - -0.7 3,085,948 + -2.6 3,085,948 + +0.2 3,086,793 + -2.7 3,086,794 - -0.0
Or see this region's nucleotide sequence