Experiment: 24hr PHYTO6 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS13490 and BPHYT_RS13495 are separated by 30 nucleotides BPHYT_RS13495 and BPHYT_RS13500 overlap by 4 nucleotides BPHYT_RS13500 and BPHYT_RS13505 overlap by 4 nucleotides
BPHYT_RS13490: BPHYT_RS13490 - hypothetical protein, at 3,078,725 to 3,079,135
_RS13490
BPHYT_RS13495: BPHYT_RS13495 - AsnC family transcriptional regulator, at 3,079,166 to 3,079,690
_RS13495
BPHYT_RS13500: BPHYT_RS13500 - AMP-dependent synthetase, at 3,079,687 to 3,081,348
_RS13500
BPHYT_RS13505: BPHYT_RS13505 - 2,4-dienoyl-CoA reductase, at 3,081,345 to 3,082,265
_RS13505
Position (kb)
3079
3080
3081
3082 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3078.908 kb on + strand, within BPHYT_RS13490 at 3078.909 kb on - strand, within BPHYT_RS13490 at 3078.981 kb on - strand, within BPHYT_RS13490 at 3079.201 kb on + strand at 3079.201 kb on + strand at 3079.202 kb on - strand at 3079.202 kb on - strand at 3079.215 kb on + strand at 3079.216 kb on - strand at 3079.216 kb on - strand at 3079.353 kb on - strand, within BPHYT_RS13495 at 3079.743 kb on + strand at 3080.238 kb on - strand, within BPHYT_RS13500 at 3080.759 kb on + strand, within BPHYT_RS13500 at 3080.759 kb on + strand, within BPHYT_RS13500 at 3080.903 kb on - strand, within BPHYT_RS13500 at 3081.051 kb on + strand, within BPHYT_RS13500 at 3081.052 kb on - strand, within BPHYT_RS13500 at 3081.052 kb on - strand, within BPHYT_RS13500 at 3081.054 kb on - strand, within BPHYT_RS13500 at 3081.054 kb on - strand, within BPHYT_RS13500 at 3081.282 kb on - strand at 3081.327 kb on - strand at 3081.327 kb on - strand at 3081.411 kb on - strand at 3081.569 kb on + strand, within BPHYT_RS13505 at 3081.588 kb on + strand, within BPHYT_RS13505 at 3081.611 kb on - strand, within BPHYT_RS13505 at 3081.815 kb on + strand, within BPHYT_RS13505 at 3081.817 kb on + strand, within BPHYT_RS13505 at 3081.818 kb on - strand, within BPHYT_RS13505 at 3081.818 kb on - strand, within BPHYT_RS13505 at 3082.312 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO6 MOI 1 remove 3,078,908 + BPHYT_RS13490 0.45 -1.0 3,078,909 - BPHYT_RS13490 0.45 -0.3 3,078,981 - BPHYT_RS13490 0.62 -0.1 3,079,201 + +1.5 3,079,201 + +0.3 3,079,202 - +0.4 3,079,202 - -0.1 3,079,215 + +1.0 3,079,216 - +0.7 3,079,216 - -2.3 3,079,353 - BPHYT_RS13495 0.36 +1.3 3,079,743 + +2.0 3,080,238 - BPHYT_RS13500 0.33 +0.3 3,080,759 + BPHYT_RS13500 0.65 +1.6 3,080,759 + BPHYT_RS13500 0.65 +0.2 3,080,903 - BPHYT_RS13500 0.73 -2.2 3,081,051 + BPHYT_RS13500 0.82 -2.2 3,081,052 - BPHYT_RS13500 0.82 -0.4 3,081,052 - BPHYT_RS13500 0.82 +0.2 3,081,054 - BPHYT_RS13500 0.82 +0.2 3,081,054 - BPHYT_RS13500 0.82 -1.0 3,081,282 - -0.6 3,081,327 - +0.1 3,081,327 - +0.1 3,081,411 - +0.4 3,081,569 + BPHYT_RS13505 0.24 +0.2 3,081,588 + BPHYT_RS13505 0.26 -1.1 3,081,611 - BPHYT_RS13505 0.29 +0.5 3,081,815 + BPHYT_RS13505 0.51 +0.4 3,081,817 + BPHYT_RS13505 0.51 -1.5 3,081,818 - BPHYT_RS13505 0.51 +0.8 3,081,818 - BPHYT_RS13505 0.51 -1.3 3,082,312 - -1.1
Or see this region's nucleotide sequence