Experiment: 24hr PHYTO6 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS13325 and BPHYT_RS13330 overlap by 23 nucleotides BPHYT_RS13330 and BPHYT_RS13335 are separated by 56 nucleotides BPHYT_RS13335 and BPHYT_RS13340 are separated by 60 nucleotides
BPHYT_RS13325: BPHYT_RS13325 - TetR family transcriptional regulator, at 3,043,322 to 3,044,032
_RS13325
BPHYT_RS13330: BPHYT_RS13330 - prevent-host-death protein, at 3,044,010 to 3,044,240
_RS13330
BPHYT_RS13335: BPHYT_RS13335 - benzoyl-CoA oxygenase, at 3,044,297 to 3,045,538
_RS13335
BPHYT_RS13340: BPHYT_RS13340 - benzoyl-CoA oxygenase, at 3,045,599 to 3,047,026
_RS13340
Position (kb) 3044
3045
3046 Strain fitness (log2 ratio) -2
-1
0
1
2 at 3043.850 kb on - strand, within BPHYT_RS13325 at 3043.875 kb on - strand, within BPHYT_RS13325 at 3044.437 kb on + strand, within BPHYT_RS13335 at 3045.414 kb on + strand at 3045.467 kb on - strand at 3045.615 kb on + strand at 3045.931 kb on - strand, within BPHYT_RS13340 at 3046.018 kb on - strand, within BPHYT_RS13340 at 3046.018 kb on - strand, within BPHYT_RS13340 at 3046.087 kb on + strand, within BPHYT_RS13340 at 3046.090 kb on - strand, within BPHYT_RS13340
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO6 MOI 1 remove 3,043,850 - BPHYT_RS13325 0.74 +0.3 3,043,875 - BPHYT_RS13325 0.78 +0.1 3,044,437 + BPHYT_RS13335 0.11 +2.1 3,045,414 + -2.6 3,045,467 - +0.9 3,045,615 + +0.7 3,045,931 - BPHYT_RS13340 0.23 -1.0 3,046,018 - BPHYT_RS13340 0.29 -0.1 3,046,018 - BPHYT_RS13340 0.29 -0.5 3,046,087 + BPHYT_RS13340 0.34 +0.8 3,046,090 - BPHYT_RS13340 0.34 -0.1
Or see this region's nucleotide sequence