Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS09850

Experiment: 24hr PHYTO6 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS09840 and BPHYT_RS09845 are separated by 32 nucleotidesBPHYT_RS09845 and BPHYT_RS09850 are separated by 34 nucleotidesBPHYT_RS09850 and BPHYT_RS09855 are separated by 199 nucleotides BPHYT_RS09840: BPHYT_RS09840 - nitrite reductase, at 2,234,815 to 2,237,355 _RS09840 BPHYT_RS09845: BPHYT_RS09845 - nitrite reductase, at 2,237,388 to 2,237,741 _RS09845 BPHYT_RS09850: BPHYT_RS09850 - molybdopterin oxidoreductase, at 2,237,776 to 2,241,987 _RS09850 BPHYT_RS09855: BPHYT_RS09855 - major facilitator superfamily protein, at 2,242,187 to 2,243,554 _RS09855 Position (kb) 2237 2238 2239 2240 2241 2242Strain fitness (log2 ratio) -1 0 1at 2236.993 kb on + strand, within BPHYT_RS09840at 2237.018 kb on - strand, within BPHYT_RS09840at 2237.168 kb on - strandat 2238.486 kb on + strand, within BPHYT_RS09850at 2238.931 kb on - strand, within BPHYT_RS09850at 2242.097 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 24hr PHYTO6 MOI 1
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2,236,993 + BPHYT_RS09840 0.86 +1.4
2,237,018 - BPHYT_RS09840 0.87 -0.8
2,237,168 - -0.7
2,238,486 + BPHYT_RS09850 0.17 +1.3
2,238,931 - BPHYT_RS09850 0.27 +0.3
2,242,097 - -0.9

Or see this region's nucleotide sequence