Experiment: 24hr PHYTO6 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS08710 and BPHYT_RS08715 overlap by 4 nucleotides BPHYT_RS08715 and BPHYT_RS08720 are separated by 150 nucleotides BPHYT_RS08720 and BPHYT_RS08725 are separated by 60 nucleotides BPHYT_RS08725 and BPHYT_RS08730 are separated by 206 nucleotides BPHYT_RS08730 and BPHYT_RS08735 are separated by 170 nucleotides
BPHYT_RS08710: BPHYT_RS08710 - hypothetical protein, at 1,977,495 to 1,979,087
_RS08710
BPHYT_RS08715: BPHYT_RS08715 - adenylyl-sulfate kinase, at 1,979,084 to 1,979,623
_RS08715
BPHYT_RS08720: BPHYT_RS08720 - membrane protein, at 1,979,774 to 1,980,400
_RS08720
BPHYT_RS08725: BPHYT_RS08725 - hypothetical protein, at 1,980,461 to 1,980,706
_RS08725
BPHYT_RS08730: BPHYT_RS08730 - hypothetical protein, at 1,980,913 to 1,981,125
_RS08730
BPHYT_RS08735: BPHYT_RS08735 - aldo-keto reductase, at 1,981,296 to 1,982,348
_RS08735
Position (kb)
1979
1980
1981 Strain fitness (log2 ratio)
-2
-1
0
1 at 1978.801 kb on - strand, within BPHYT_RS08710 at 1978.947 kb on + strand at 1978.948 kb on - strand at 1979.422 kb on - strand, within BPHYT_RS08715 at 1979.457 kb on - strand, within BPHYT_RS08715 at 1980.020 kb on - strand, within BPHYT_RS08720 at 1980.239 kb on + strand, within BPHYT_RS08720 at 1980.239 kb on + strand, within BPHYT_RS08720 at 1980.239 kb on + strand, within BPHYT_RS08720 at 1980.240 kb on - strand, within BPHYT_RS08720 at 1980.240 kb on - strand, within BPHYT_RS08720 at 1980.240 kb on - strand, within BPHYT_RS08720 at 1980.366 kb on - strand at 1980.769 kb on - strand at 1980.819 kb on - strand at 1980.855 kb on - strand at 1980.855 kb on - strand at 1980.855 kb on - strand at 1981.034 kb on + strand, within BPHYT_RS08730 at 1981.035 kb on - strand, within BPHYT_RS08730 at 1981.035 kb on - strand, within BPHYT_RS08730 at 1981.035 kb on - strand, within BPHYT_RS08730 at 1981.208 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO6 MOI 1 remove 1,978,801 - BPHYT_RS08710 0.82 +0.7 1,978,947 + +0.9 1,978,948 - -0.8 1,979,422 - BPHYT_RS08715 0.63 -0.8 1,979,457 - BPHYT_RS08715 0.69 +0.6 1,980,020 - BPHYT_RS08720 0.39 -1.3 1,980,239 + BPHYT_RS08720 0.74 +0.4 1,980,239 + BPHYT_RS08720 0.74 +0.2 1,980,239 + BPHYT_RS08720 0.74 +0.3 1,980,240 - BPHYT_RS08720 0.74 +0.2 1,980,240 - BPHYT_RS08720 0.74 -2.1 1,980,240 - BPHYT_RS08720 0.74 +0.3 1,980,366 - +1.4 1,980,769 - +1.6 1,980,819 - -2.0 1,980,855 - -2.1 1,980,855 - -1.6 1,980,855 - -0.4 1,981,034 + BPHYT_RS08730 0.57 +0.2 1,981,035 - BPHYT_RS08730 0.57 -0.3 1,981,035 - BPHYT_RS08730 0.57 -1.3 1,981,035 - BPHYT_RS08730 0.57 -1.5 1,981,208 - -0.6
Or see this region's nucleotide sequence