Experiment: 24hr PHYTO6 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS03315 and BPHYT_RS03320 are separated by 87 nucleotides BPHYT_RS03320 and BPHYT_RS03325 are separated by 25 nucleotides BPHYT_RS03325 and BPHYT_RS03330 are separated by 79 nucleotides
BPHYT_RS03315: BPHYT_RS03315 - polymerase, at 747,186 to 748,307
_RS03315
BPHYT_RS03320: BPHYT_RS03320 - lactoylglutathione lyase, at 748,395 to 748,781
_RS03320
BPHYT_RS03325: BPHYT_RS03325 - hypothetical protein, at 748,807 to 749,205
_RS03325
BPHYT_RS03330: BPHYT_RS03330 - hypothetical protein, at 749,285 to 750,187
_RS03330
Position (kb) 748
749 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 747.422 kb on + strand, within BPHYT_RS03315 at 747.422 kb on + strand, within BPHYT_RS03315 at 747.423 kb on - strand, within BPHYT_RS03315 at 747.423 kb on - strand, within BPHYT_RS03315 at 747.450 kb on + strand, within BPHYT_RS03315 at 747.451 kb on - strand, within BPHYT_RS03315 at 747.552 kb on + strand, within BPHYT_RS03315 at 747.678 kb on + strand, within BPHYT_RS03315 at 747.678 kb on + strand, within BPHYT_RS03315 at 747.690 kb on + strand, within BPHYT_RS03315 at 747.690 kb on + strand, within BPHYT_RS03315 at 747.777 kb on + strand, within BPHYT_RS03315 at 747.880 kb on - strand, within BPHYT_RS03315 at 747.912 kb on + strand, within BPHYT_RS03315 at 747.912 kb on + strand, within BPHYT_RS03315 at 747.912 kb on + strand, within BPHYT_RS03315 at 747.912 kb on + strand, within BPHYT_RS03315 at 747.912 kb on + strand, within BPHYT_RS03315 at 747.913 kb on - strand, within BPHYT_RS03315 at 747.913 kb on - strand, within BPHYT_RS03315 at 747.913 kb on - strand, within BPHYT_RS03315 at 747.915 kb on - strand, within BPHYT_RS03315 at 747.993 kb on + strand, within BPHYT_RS03315 at 747.993 kb on + strand, within BPHYT_RS03315 at 748.131 kb on + strand, within BPHYT_RS03315 at 748.131 kb on + strand, within BPHYT_RS03315 at 748.150 kb on - strand, within BPHYT_RS03315 at 748.177 kb on - strand, within BPHYT_RS03315 at 748.290 kb on - strand at 748.290 kb on - strand at 748.373 kb on - strand at 748.452 kb on + strand, within BPHYT_RS03320 at 748.505 kb on + strand, within BPHYT_RS03320 at 748.591 kb on - strand, within BPHYT_RS03320 at 748.749 kb on + strand at 748.780 kb on - strand at 748.813 kb on - strand at 749.057 kb on - strand, within BPHYT_RS03325 at 749.089 kb on + strand, within BPHYT_RS03325 at 749.130 kb on + strand, within BPHYT_RS03325 at 749.265 kb on + strand at 749.565 kb on + strand, within BPHYT_RS03330
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO6 MOI 1 remove 747,422 + BPHYT_RS03315 0.21 +0.7 747,422 + BPHYT_RS03315 0.21 -0.3 747,423 - BPHYT_RS03315 0.21 -1.1 747,423 - BPHYT_RS03315 0.21 -1.2 747,450 + BPHYT_RS03315 0.24 +0.3 747,451 - BPHYT_RS03315 0.24 -0.6 747,552 + BPHYT_RS03315 0.33 +3.8 747,678 + BPHYT_RS03315 0.44 -0.5 747,678 + BPHYT_RS03315 0.44 -0.2 747,690 + BPHYT_RS03315 0.45 +1.8 747,690 + BPHYT_RS03315 0.45 -0.1 747,777 + BPHYT_RS03315 0.53 +1.3 747,880 - BPHYT_RS03315 0.62 -0.1 747,912 + BPHYT_RS03315 0.65 -0.5 747,912 + BPHYT_RS03315 0.65 -0.3 747,912 + BPHYT_RS03315 0.65 -1.1 747,912 + BPHYT_RS03315 0.65 -1.1 747,912 + BPHYT_RS03315 0.65 +1.1 747,913 - BPHYT_RS03315 0.65 -0.5 747,913 - BPHYT_RS03315 0.65 -0.3 747,913 - BPHYT_RS03315 0.65 -3.0 747,915 - BPHYT_RS03315 0.65 -2.8 747,993 + BPHYT_RS03315 0.72 -0.2 747,993 + BPHYT_RS03315 0.72 -0.1 748,131 + BPHYT_RS03315 0.84 +1.3 748,131 + BPHYT_RS03315 0.84 -1.0 748,150 - BPHYT_RS03315 0.86 +0.1 748,177 - BPHYT_RS03315 0.88 -0.7 748,290 - +0.4 748,290 - -0.9 748,373 - -0.3 748,452 + BPHYT_RS03320 0.15 -1.4 748,505 + BPHYT_RS03320 0.28 +0.0 748,591 - BPHYT_RS03320 0.51 -0.6 748,749 + +2.3 748,780 - -1.1 748,813 - +1.6 749,057 - BPHYT_RS03325 0.63 -2.9 749,089 + BPHYT_RS03325 0.71 -1.0 749,130 + BPHYT_RS03325 0.81 -0.2 749,265 + -1.5 749,565 + BPHYT_RS03330 0.31 -1.3
Or see this region's nucleotide sequence