Experiment: 24hr PHYTO6 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS02220 and BPHYT_RS02225 are separated by 27 nucleotides BPHYT_RS02225 and BPHYT_RS02230 are separated by 47 nucleotides BPHYT_RS02230 and BPHYT_RS02235 overlap by 8 nucleotides BPHYT_RS02235 and BPHYT_RS02240 are separated by 94 nucleotides
BPHYT_RS02220: BPHYT_RS02220 - butyryl-CoA dehydrogenase, at 510,218 to 511,348
_RS02220
BPHYT_RS02225: BPHYT_RS02225 - short-chain dehydrogenase, at 511,376 to 512,053
_RS02225
BPHYT_RS02230: BPHYT_RS02230 - acetyl-CoA acetyltransferase, at 512,101 to 513,294
_RS02230
BPHYT_RS02235: BPHYT_RS02235 - beta carbonic anhydrase, at 513,287 to 514,063
_RS02235
BPHYT_RS02240: BPHYT_RS02240 - isocitrate dehydrogenase kinase/phosphatase, at 514,158 to 515,996
_RS02240
Position (kb) 512
513
514 Strain fitness (log2 ratio) -1
0
1
2 at 511.402 kb on - strand at 511.407 kb on + strand at 511.993 kb on - strand at 511.993 kb on - strand at 512.056 kb on - strand at 512.056 kb on - strand at 512.056 kb on - strand at 512.058 kb on + strand at 512.058 kb on + strand at 512.342 kb on + strand, within BPHYT_RS02230 at 512.825 kb on + strand, within BPHYT_RS02230 at 512.846 kb on + strand, within BPHYT_RS02230 at 512.912 kb on + strand, within BPHYT_RS02230 at 513.424 kb on - strand, within BPHYT_RS02235 at 513.496 kb on - strand, within BPHYT_RS02235 at 513.554 kb on - strand, within BPHYT_RS02235 at 513.564 kb on - strand, within BPHYT_RS02235 at 514.113 kb on + strand at 514.114 kb on - strand at 514.114 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO6 MOI 1 remove 511,402 - +0.4 511,407 + -0.4 511,993 - -1.4 511,993 - -0.5 512,056 - +0.0 512,056 - -0.3 512,056 - +0.2 512,058 + +2.2 512,058 + +0.4 512,342 + BPHYT_RS02230 0.20 +2.0 512,825 + BPHYT_RS02230 0.61 +1.5 512,846 + BPHYT_RS02230 0.62 -0.6 512,912 + BPHYT_RS02230 0.68 +0.2 513,424 - BPHYT_RS02235 0.18 -0.0 513,496 - BPHYT_RS02235 0.27 +0.7 513,554 - BPHYT_RS02235 0.34 +0.4 513,564 - BPHYT_RS02235 0.36 +0.0 514,113 + +0.5 514,114 - -1.6 514,114 - -0.4
Or see this region's nucleotide sequence