Experiment: 24hr PHYTO6 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS00790 and BPHYT_RS00795 are separated by 189 nucleotides BPHYT_RS00795 and BPHYT_RS00800 are separated by 14 nucleotides BPHYT_RS00800 and BPHYT_RS00805 overlap by 8 nucleotides
BPHYT_RS00790: BPHYT_RS00790 - conjugal transfer protein TraF, at 179,432 to 180,142
_RS00790
BPHYT_RS00795: BPHYT_RS00795 - conjugal transfer protein TraJ, at 180,332 to 181,090
_RS00795
BPHYT_RS00800: BPHYT_RS00800 - Forms the bulk of type IV secretion complex that spans outer membrane and periplasm (VirB9), at 181,105 to 181,932
_RS00800
BPHYT_RS00805: BPHYT_RS00805 - type IV secretion system protein VirB10, at 181,925 to 183,127
_RS00805
Position (kb) 181
182 Strain fitness (log2 ratio) -2
-1
0
1
2 at 180.258 kb on - strand at 180.518 kb on + strand at 180.519 kb on - strand, within BPHYT_RS00795 at 180.642 kb on - strand, within BPHYT_RS00795 at 180.663 kb on - strand, within BPHYT_RS00795 at 180.746 kb on + strand, within BPHYT_RS00795 at 180.746 kb on + strand, within BPHYT_RS00795 at 181.005 kb on - strand, within BPHYT_RS00795 at 181.142 kb on + strand at 181.143 kb on - strand at 181.171 kb on + strand at 181.172 kb on - strand at 181.366 kb on + strand, within BPHYT_RS00800 at 181.366 kb on + strand, within BPHYT_RS00800 at 181.367 kb on - strand, within BPHYT_RS00800 at 181.430 kb on - strand, within BPHYT_RS00800 at 181.489 kb on + strand, within BPHYT_RS00800 at 181.490 kb on - strand, within BPHYT_RS00800 at 181.490 kb on - strand, within BPHYT_RS00800 at 181.597 kb on - strand, within BPHYT_RS00800 at 181.603 kb on + strand, within BPHYT_RS00800 at 181.676 kb on - strand, within BPHYT_RS00800 at 182.182 kb on - strand, within BPHYT_RS00805 at 182.242 kb on + strand at 182.292 kb on + strand, within BPHYT_RS00805 at 182.339 kb on + strand, within BPHYT_RS00805 at 182.340 kb on - strand, within BPHYT_RS00805 at 182.340 kb on - strand, within BPHYT_RS00805 at 182.343 kb on - strand, within BPHYT_RS00805 at 182.635 kb on + strand, within BPHYT_RS00805 at 182.844 kb on - strand, within BPHYT_RS00805 at 182.844 kb on - strand, within BPHYT_RS00805
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO6 MOI 1 remove 180,258 - +0.1 180,518 + +0.8 180,519 - BPHYT_RS00795 0.25 +2.3 180,642 - BPHYT_RS00795 0.41 -0.7 180,663 - BPHYT_RS00795 0.44 +1.3 180,746 + BPHYT_RS00795 0.55 +0.7 180,746 + BPHYT_RS00795 0.55 -0.2 181,005 - BPHYT_RS00795 0.89 -1.2 181,142 + -0.2 181,143 - +0.6 181,171 + +0.2 181,172 - +0.1 181,366 + BPHYT_RS00800 0.32 -0.3 181,366 + BPHYT_RS00800 0.32 +0.2 181,367 - BPHYT_RS00800 0.32 -0.2 181,430 - BPHYT_RS00800 0.39 -1.0 181,489 + BPHYT_RS00800 0.46 -0.1 181,490 - BPHYT_RS00800 0.46 -1.1 181,490 - BPHYT_RS00800 0.46 -0.8 181,597 - BPHYT_RS00800 0.59 -0.7 181,603 + BPHYT_RS00800 0.60 -0.1 181,676 - BPHYT_RS00800 0.69 +0.1 182,182 - BPHYT_RS00805 0.21 -1.1 182,242 + -0.2 182,292 + BPHYT_RS00805 0.31 -0.2 182,339 + BPHYT_RS00805 0.34 +0.1 182,340 - BPHYT_RS00805 0.34 +0.1 182,340 - BPHYT_RS00805 0.34 +0.2 182,343 - BPHYT_RS00805 0.35 -2.4 182,635 + BPHYT_RS00805 0.59 -0.7 182,844 - BPHYT_RS00805 0.76 +1.3 182,844 - BPHYT_RS00805 0.76 -0.4
Or see this region's nucleotide sequence