Experiment: 24hr PHYTO6 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS00785 and BPHYT_RS00790 overlap by 4 nucleotides BPHYT_RS00790 and BPHYT_RS00795 are separated by 189 nucleotides BPHYT_RS00795 and BPHYT_RS00800 are separated by 14 nucleotides
BPHYT_RS00785: BPHYT_RS00785 - conjugal transfer protein TraH, at 178,278 to 179,435
_RS00785
BPHYT_RS00790: BPHYT_RS00790 - conjugal transfer protein TraF, at 179,432 to 180,142
_RS00790
BPHYT_RS00795: BPHYT_RS00795 - conjugal transfer protein TraJ, at 180,332 to 181,090
_RS00795
BPHYT_RS00800: BPHYT_RS00800 - Forms the bulk of type IV secretion complex that spans outer membrane and periplasm (VirB9), at 181,105 to 181,932
_RS00800
Position (kb) 179
180
181 Strain fitness (log2 ratio) -2
-1
0
1
2 at 178.521 kb on + strand, within BPHYT_RS00785 at 178.521 kb on + strand, within BPHYT_RS00785 at 178.544 kb on - strand, within BPHYT_RS00785 at 178.671 kb on + strand, within BPHYT_RS00785 at 178.723 kb on - strand, within BPHYT_RS00785 at 178.969 kb on - strand, within BPHYT_RS00785 at 179.257 kb on - strand, within BPHYT_RS00785 at 179.381 kb on + strand at 179.532 kb on - strand, within BPHYT_RS00790 at 179.702 kb on - strand, within BPHYT_RS00790 at 179.702 kb on - strand, within BPHYT_RS00790 at 180.258 kb on - strand at 180.518 kb on + strand at 180.519 kb on - strand, within BPHYT_RS00795 at 180.642 kb on - strand, within BPHYT_RS00795 at 180.663 kb on - strand, within BPHYT_RS00795 at 180.746 kb on + strand, within BPHYT_RS00795 at 180.746 kb on + strand, within BPHYT_RS00795 at 181.005 kb on - strand, within BPHYT_RS00795 at 181.142 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO6 MOI 1 remove 178,521 + BPHYT_RS00785 0.21 -1.1 178,521 + BPHYT_RS00785 0.21 -1.0 178,544 - BPHYT_RS00785 0.23 -0.1 178,671 + BPHYT_RS00785 0.34 +0.0 178,723 - BPHYT_RS00785 0.38 -0.1 178,969 - BPHYT_RS00785 0.60 +0.3 179,257 - BPHYT_RS00785 0.85 -0.5 179,381 + -0.6 179,532 - BPHYT_RS00790 0.14 -2.3 179,702 - BPHYT_RS00790 0.38 +0.9 179,702 - BPHYT_RS00790 0.38 +1.4 180,258 - +0.1 180,518 + +0.8 180,519 - BPHYT_RS00795 0.25 +2.3 180,642 - BPHYT_RS00795 0.41 -0.7 180,663 - BPHYT_RS00795 0.44 +1.3 180,746 + BPHYT_RS00795 0.55 +0.7 180,746 + BPHYT_RS00795 0.55 -0.2 181,005 - BPHYT_RS00795 0.89 -1.2 181,142 + -0.2
Or see this region's nucleotide sequence