Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS29755

Experiment: 24hr PHYTO6 MOI 10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS29750 and BPHYT_RS29755 are separated by 603 nucleotides BPHYT_RS29750: BPHYT_RS29750 - phosphoenolpyruvate phosphomutase, at 2,227,181 to 2,228,926 _RS29750 BPHYT_RS29755: BPHYT_RS29755 - membrane protein, at 2,229,530 to 2,231,071 _RS29755 Position (kb) 2229 2230 2231 2232Strain fitness (log2 ratio) -2 -1 0 1 2 3 4at 2228.749 kb on - strand, within BPHYT_RS29750at 2228.749 kb on - strand, within BPHYT_RS29750at 2228.929 kb on + strandat 2228.929 kb on + strandat 2228.933 kb on + strandat 2228.934 kb on - strandat 2228.934 kb on - strandat 2228.965 kb on + strandat 2228.966 kb on - strandat 2229.451 kb on + strandat 2229.693 kb on - strand, within BPHYT_RS29755at 2229.693 kb on - strand, within BPHYT_RS29755at 2229.788 kb on + strand, within BPHYT_RS29755at 2229.947 kb on + strand, within BPHYT_RS29755at 2229.947 kb on + strand, within BPHYT_RS29755at 2229.947 kb on + strand, within BPHYT_RS29755at 2229.948 kb on - strand, within BPHYT_RS29755at 2229.996 kb on - strand, within BPHYT_RS29755at 2230.025 kb on + strand, within BPHYT_RS29755at 2230.043 kb on + strand, within BPHYT_RS29755at 2230.043 kb on + strand, within BPHYT_RS29755at 2230.043 kb on + strand, within BPHYT_RS29755at 2230.043 kb on + strand, within BPHYT_RS29755at 2230.044 kb on - strand, within BPHYT_RS29755at 2230.044 kb on - strand, within BPHYT_RS29755at 2230.044 kb on - strand, within BPHYT_RS29755at 2230.044 kb on - strand, within BPHYT_RS29755at 2230.099 kb on + strand, within BPHYT_RS29755at 2230.099 kb on + strand, within BPHYT_RS29755at 2230.154 kb on - strand, within BPHYT_RS29755at 2231.338 kb on - strandat 2231.338 kb on - strandat 2231.502 kb on + strandat 2231.680 kb on + strandat 2231.683 kb on + strandat 2231.684 kb on - strandat 2231.684 kb on - strandat 2231.960 kb on + strandat 2231.960 kb on + strandat 2231.962 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 24hr PHYTO6 MOI 10
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2,228,749 - BPHYT_RS29750 0.90 +2.3
2,228,749 - BPHYT_RS29750 0.90 -0.9
2,228,929 + +0.3
2,228,929 + +1.6
2,228,933 + +3.2
2,228,934 - -0.2
2,228,934 - -1.0
2,228,965 + +1.1
2,228,966 - -0.4
2,229,451 + +1.3
2,229,693 - BPHYT_RS29755 0.11 +1.3
2,229,693 - BPHYT_RS29755 0.11 -2.4
2,229,788 + BPHYT_RS29755 0.17 -2.4
2,229,947 + BPHYT_RS29755 0.27 +3.2
2,229,947 + BPHYT_RS29755 0.27 +0.2
2,229,947 + BPHYT_RS29755 0.27 -0.3
2,229,948 - BPHYT_RS29755 0.27 +3.9
2,229,996 - BPHYT_RS29755 0.30 +2.5
2,230,025 + BPHYT_RS29755 0.32 +0.6
2,230,043 + BPHYT_RS29755 0.33 +0.7
2,230,043 + BPHYT_RS29755 0.33 -0.1
2,230,043 + BPHYT_RS29755 0.33 -0.5
2,230,043 + BPHYT_RS29755 0.33 +0.7
2,230,044 - BPHYT_RS29755 0.33 -1.0
2,230,044 - BPHYT_RS29755 0.33 +1.9
2,230,044 - BPHYT_RS29755 0.33 -0.0
2,230,044 - BPHYT_RS29755 0.33 -0.9
2,230,099 + BPHYT_RS29755 0.37 +1.4
2,230,099 + BPHYT_RS29755 0.37 -1.4
2,230,154 - BPHYT_RS29755 0.40 +0.5
2,231,338 - +1.0
2,231,338 - +0.0
2,231,502 + +0.1
2,231,680 + +0.3
2,231,683 + -0.5
2,231,684 - +1.4
2,231,684 - +0.4
2,231,960 + -1.5
2,231,960 + +1.3
2,231,962 + +0.6

Or see this region's nucleotide sequence