Experiment: 24hr PHYTO6 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS29710 and BPHYT_RS29715 overlap by 4 nucleotides BPHYT_RS29715 and BPHYT_RS29720 are separated by 124 nucleotides BPHYT_RS29720 and BPHYT_RS29725 are separated by 96 nucleotides
BPHYT_RS29710: BPHYT_RS29710 - FAD-binding molybdopterin dehydrogenase, at 2,216,909 to 2,217,910
_RS29710
BPHYT_RS29715: BPHYT_RS29715 - acylaldehyde oxidase, at 2,217,907 to 2,220,129
_RS29715
BPHYT_RS29720: BPHYT_RS29720 - molybdopterin-guanine dinucleotide biosynthesis protein MobA, at 2,220,254 to 2,220,841
_RS29720
BPHYT_RS29725: BPHYT_RS29725 - aromatic compound catabolic protein, at 2,220,938 to 2,221,486
_RS29725
Position (kb)
2217
2218
2219
2220
2221 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2217.593 kb on + strand, within BPHYT_RS29710 at 2217.621 kb on - strand, within BPHYT_RS29710 at 2218.429 kb on + strand, within BPHYT_RS29715 at 2218.874 kb on - strand, within BPHYT_RS29715 at 2219.104 kb on + strand, within BPHYT_RS29715 at 2219.308 kb on + strand, within BPHYT_RS29715 at 2219.308 kb on + strand, within BPHYT_RS29715 at 2219.850 kb on - strand, within BPHYT_RS29715 at 2219.871 kb on + strand, within BPHYT_RS29715 at 2219.953 kb on - strand at 2220.003 kb on + strand at 2220.056 kb on - strand at 2220.107 kb on + strand at 2220.239 kb on + strand at 2220.335 kb on - strand, within BPHYT_RS29720 at 2220.659 kb on + strand, within BPHYT_RS29720 at 2220.869 kb on - strand at 2220.892 kb on - strand at 2220.946 kb on + strand at 2220.990 kb on + strand at 2221.108 kb on - strand, within BPHYT_RS29725 at 2221.108 kb on - strand, within BPHYT_RS29725
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO6 MOI 10 remove 2,217,593 + BPHYT_RS29710 0.68 -0.7 2,217,621 - BPHYT_RS29710 0.71 +0.7 2,218,429 + BPHYT_RS29715 0.23 -1.2 2,218,874 - BPHYT_RS29715 0.43 -0.5 2,219,104 + BPHYT_RS29715 0.54 +1.3 2,219,308 + BPHYT_RS29715 0.63 +0.1 2,219,308 + BPHYT_RS29715 0.63 -0.6 2,219,850 - BPHYT_RS29715 0.87 -0.5 2,219,871 + BPHYT_RS29715 0.88 -1.4 2,219,953 - -3.0 2,220,003 + -1.0 2,220,056 - -0.0 2,220,107 + -1.1 2,220,239 + +0.5 2,220,335 - BPHYT_RS29720 0.14 +2.0 2,220,659 + BPHYT_RS29720 0.69 +0.4 2,220,869 - +0.3 2,220,892 - +0.7 2,220,946 + -1.5 2,220,990 + +1.4 2,221,108 - BPHYT_RS29725 0.31 -1.2 2,221,108 - BPHYT_RS29725 0.31 +2.3
Or see this region's nucleotide sequence