Experiment: 24hr PHYTO6 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS28500 and BPHYT_RS28505 are separated by 16 nucleotides BPHYT_RS28505 and BPHYT_RS28510 are separated by 69 nucleotides BPHYT_RS28510 and BPHYT_RS28515 overlap by 14 nucleotides
BPHYT_RS28500: BPHYT_RS28500 - LysR family transcriptional regulator, at 1,948,947 to 1,949,903
_RS28500
BPHYT_RS28505: BPHYT_RS28505 - 5-deoxyglucuronate isomerase, at 1,949,920 to 1,950,750
_RS28505
BPHYT_RS28510: BPHYT_RS28510 - dioxygenase, at 1,950,820 to 1,952,298
_RS28510
BPHYT_RS28515: BPHYT_RS28515 - mannonate dehydratase, at 1,952,285 to 1,953,337
_RS28515
Position (kb) 1950
1951
1952
1953 Strain fitness (log2 ratio) -2
-1
0
1
2 at 1950.033 kb on - strand, within BPHYT_RS28505 at 1950.041 kb on + strand, within BPHYT_RS28505 at 1950.186 kb on - strand, within BPHYT_RS28505 at 1951.283 kb on - strand, within BPHYT_RS28510 at 1951.377 kb on - strand, within BPHYT_RS28510 at 1951.427 kb on + strand, within BPHYT_RS28510 at 1951.640 kb on - strand, within BPHYT_RS28510 at 1951.893 kb on - strand, within BPHYT_RS28510 at 1952.154 kb on - strand at 1953.185 kb on + strand, within BPHYT_RS28515 at 1953.185 kb on + strand, within BPHYT_RS28515 at 1953.186 kb on - strand, within BPHYT_RS28515 at 1953.247 kb on - strand at 1953.291 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO6 MOI 10 remove 1,950,033 - BPHYT_RS28505 0.14 +1.9 1,950,041 + BPHYT_RS28505 0.15 -2.5 1,950,186 - BPHYT_RS28505 0.32 -1.5 1,951,283 - BPHYT_RS28510 0.31 -2.3 1,951,377 - BPHYT_RS28510 0.38 +0.2 1,951,427 + BPHYT_RS28510 0.41 +0.4 1,951,640 - BPHYT_RS28510 0.55 -1.8 1,951,893 - BPHYT_RS28510 0.73 -1.1 1,952,154 - -1.0 1,953,185 + BPHYT_RS28515 0.85 -1.1 1,953,185 + BPHYT_RS28515 0.85 +2.0 1,953,186 - BPHYT_RS28515 0.86 -0.5 1,953,247 - +0.3 1,953,291 + -1.1
Or see this region's nucleotide sequence