Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS27985

Experiment: 24hr PHYTO6 MOI 10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS27980 and BPHYT_RS27985 are separated by 114 nucleotidesBPHYT_RS27985 and BPHYT_RS27990 are separated by 473 nucleotides BPHYT_RS27980: BPHYT_RS27980 - ABC transporter substrate-binding protein, at 1,841,528 to 1,842,859 _RS27980 BPHYT_RS27985: BPHYT_RS27985 - LacI family transcriptional regulator, at 1,842,974 to 1,843,978 _RS27985 BPHYT_RS27990: BPHYT_RS27990 - hypothetical protein, at 1,844,452 to 1,844,763 _RS27990 Position (kb) 1842 1843 1844Strain fitness (log2 ratio) -2 -1 0 1 2at 1842.151 kb on - strand, within BPHYT_RS27980at 1842.257 kb on + strand, within BPHYT_RS27980at 1843.516 kb on - strand, within BPHYT_RS27985at 1843.516 kb on - strand, within BPHYT_RS27985at 1843.702 kb on - strand, within BPHYT_RS27985at 1843.824 kb on - strand, within BPHYT_RS27985at 1843.824 kb on - strand, within BPHYT_RS27985at 1843.978 kb on + strandat 1843.978 kb on + strandat 1843.978 kb on + strandat 1844.357 kb on + strandat 1844.771 kb on - strandat 1844.831 kb on + strandat 1844.836 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 24hr PHYTO6 MOI 10
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1,842,151 - BPHYT_RS27980 0.47 +0.7
1,842,257 + BPHYT_RS27980 0.55 +0.8
1,843,516 - BPHYT_RS27985 0.54 -0.8
1,843,516 - BPHYT_RS27985 0.54 -0.2
1,843,702 - BPHYT_RS27985 0.72 -0.7
1,843,824 - BPHYT_RS27985 0.85 -1.0
1,843,824 - BPHYT_RS27985 0.85 -0.7
1,843,978 + -2.4
1,843,978 + +1.3
1,843,978 + -0.5
1,844,357 + +1.5
1,844,771 - +0.2
1,844,831 + +2.3
1,844,836 - -0.4

Or see this region's nucleotide sequence