Experiment: 24hr PHYTO6 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS26445 and BPHYT_RS26450 overlap by 4 nucleotides BPHYT_RS26450 and BPHYT_RS26455 are separated by 195 nucleotides BPHYT_RS26455 and BPHYT_RS26460 are separated by 165 nucleotides
BPHYT_RS26445: BPHYT_RS26445 - NADPH:quinone reductase, at 1,504,945 to 1,505,934
_RS26445
BPHYT_RS26450: BPHYT_RS26450 - transcriptional regulator, at 1,505,931 to 1,506,872
_RS26450
BPHYT_RS26455: BPHYT_RS26455 - hypothetical protein, at 1,507,068 to 1,507,505
_RS26455
BPHYT_RS26460: BPHYT_RS26460 - hypothetical protein, at 1,507,671 to 1,508,015
_RS26460
Position (kb)
1505
1506
1507 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1505.098 kb on + strand, within BPHYT_RS26445 at 1505.098 kb on + strand, within BPHYT_RS26445 at 1505.098 kb on + strand, within BPHYT_RS26445 at 1505.234 kb on - strand, within BPHYT_RS26445 at 1505.242 kb on + strand, within BPHYT_RS26445 at 1505.446 kb on + strand, within BPHYT_RS26445 at 1505.446 kb on + strand, within BPHYT_RS26445 at 1505.665 kb on + strand, within BPHYT_RS26445 at 1505.671 kb on + strand, within BPHYT_RS26445 at 1505.672 kb on - strand, within BPHYT_RS26445 at 1505.777 kb on + strand, within BPHYT_RS26445 at 1506.298 kb on + strand, within BPHYT_RS26450 at 1506.361 kb on + strand, within BPHYT_RS26450 at 1506.892 kb on - strand at 1506.936 kb on + strand at 1507.644 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO6 MOI 10 remove 1,505,098 + BPHYT_RS26445 0.15 -2.9 1,505,098 + BPHYT_RS26445 0.15 -1.1 1,505,098 + BPHYT_RS26445 0.15 +1.1 1,505,234 - BPHYT_RS26445 0.29 -0.5 1,505,242 + BPHYT_RS26445 0.30 -1.4 1,505,446 + BPHYT_RS26445 0.51 +2.4 1,505,446 + BPHYT_RS26445 0.51 +0.1 1,505,665 + BPHYT_RS26445 0.73 +2.3 1,505,671 + BPHYT_RS26445 0.73 +0.3 1,505,672 - BPHYT_RS26445 0.73 -1.8 1,505,777 + BPHYT_RS26445 0.84 +1.1 1,506,298 + BPHYT_RS26450 0.39 -1.4 1,506,361 + BPHYT_RS26450 0.46 -1.4 1,506,892 - +0.7 1,506,936 + +0.1 1,507,644 + +0.5
Or see this region's nucleotide sequence