Experiment: 24hr PHYTO6 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS26015 and BPHYT_RS26020 are separated by 35 nucleotides BPHYT_RS26020 and BPHYT_RS26025 are separated by 35 nucleotides BPHYT_RS26025 and BPHYT_RS26030 overlap by 10 nucleotides BPHYT_RS26030 and BPHYT_RS26035 overlap by 4 nucleotides
BPHYT_RS26015: BPHYT_RS26015 - rod shape-determining protein MreB, at 1,412,856 to 1,413,899
_RS26015
BPHYT_RS26020: BPHYT_RS26020 - GntR family transcriptional regulator, at 1,413,935 to 1,414,693
_RS26020
BPHYT_RS26025: BPHYT_RS26025 - hypothetical protein, at 1,414,729 to 1,415,124
_RS26025
BPHYT_RS26030: BPHYT_RS26030 - cysteine hydrolase, at 1,415,115 to 1,415,810
_RS26030
BPHYT_RS26035: BPHYT_RS26035 - ABC transporter ATP-binding protein, at 1,415,807 to 1,417,417
_RS26035
Position (kb) 1414
1415
1416 Strain fitness (log2 ratio) -1
0
1
2
3 at 1413.891 kb on + strand at 1414.372 kb on + strand, within BPHYT_RS26020 at 1415.070 kb on - strand, within BPHYT_RS26025 at 1415.236 kb on + strand, within BPHYT_RS26030 at 1415.588 kb on + strand, within BPHYT_RS26030 at 1415.588 kb on + strand, within BPHYT_RS26030
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO6 MOI 10 remove 1,413,891 + -1.4 1,414,372 + BPHYT_RS26020 0.58 -0.3 1,415,070 - BPHYT_RS26025 0.86 +1.8 1,415,236 + BPHYT_RS26030 0.17 +3.3 1,415,588 + BPHYT_RS26030 0.68 +0.6 1,415,588 + BPHYT_RS26030 0.68 +0.3
Or see this region's nucleotide sequence