Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS25810

Experiment: 24hr PHYTO6 MOI 10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS25805 and BPHYT_RS25810 are separated by 182 nucleotidesBPHYT_RS25810 and BPHYT_RS25815 are separated by 16 nucleotides BPHYT_RS25805: BPHYT_RS25805 - deoxyribose kinase (EC 2.7.1.15) (from data), at 1,362,000 to 1,362,986 _RS25805 BPHYT_RS25810: BPHYT_RS25810 - acetaldehyde dehydrogenase (EC 1.2.1.3) (from data), at 1,363,169 to 1,365,559 _RS25810 BPHYT_RS25815: BPHYT_RS25815 - deoxyribose-phosphate aldolase, at 1,365,576 to 1,366,586 _RS25815 Position (kb) 1363 1364 1365 1366Strain fitness (log2 ratio) -2 -1 0 1at 1362.242 kb on - strand, within BPHYT_RS25805at 1362.421 kb on + strand, within BPHYT_RS25805at 1362.931 kb on + strandat 1362.931 kb on + strandat 1362.932 kb on - strandat 1363.050 kb on + strandat 1363.051 kb on - strandat 1363.217 kb on + strandat 1363.458 kb on + strand, within BPHYT_RS25810at 1363.678 kb on - strand, within BPHYT_RS25810at 1363.801 kb on - strand, within BPHYT_RS25810at 1363.951 kb on - strand, within BPHYT_RS25810at 1365.236 kb on - strand, within BPHYT_RS25810at 1366.048 kb on + strand, within BPHYT_RS25815at 1366.223 kb on - strand, within BPHYT_RS25815

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Per-strain Table

Position Strand Gene LocusTag Fraction 24hr PHYTO6 MOI 10
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1,362,242 - BPHYT_RS25805 0.25 -0.0
1,362,421 + BPHYT_RS25805 0.43 -0.9
1,362,931 + -0.3
1,362,931 + +0.4
1,362,932 - -0.9
1,363,050 + -2.1
1,363,051 - -1.5
1,363,217 + -0.9
1,363,458 + BPHYT_RS25810 0.12 -1.2
1,363,678 - BPHYT_RS25810 0.21 -0.7
1,363,801 - BPHYT_RS25810 0.26 +0.3
1,363,951 - BPHYT_RS25810 0.33 -0.5
1,365,236 - BPHYT_RS25810 0.86 -0.8
1,366,048 + BPHYT_RS25815 0.47 -2.4
1,366,223 - BPHYT_RS25815 0.64 -0.3

Or see this region's nucleotide sequence