Experiment: 24hr PHYTO6 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS23645 and BPHYT_RS23650 are separated by 160 nucleotides BPHYT_RS23650 and BPHYT_RS23655 are separated by 90 nucleotides BPHYT_RS23655 and BPHYT_RS23660 are separated by 60 nucleotides
BPHYT_RS23645: BPHYT_RS23645 - phosphohydrolase, at 875,586 to 876,230
_RS23645
BPHYT_RS23650: BPHYT_RS23650 - hypothetical protein, at 876,391 to 876,729
_RS23650
BPHYT_RS23655: BPHYT_RS23655 - peroxiredoxin, at 876,820 to 877,296
_RS23655
BPHYT_RS23660: BPHYT_RS23660 - manganese transporter, at 877,357 to 879,015
_RS23660
Position (kb) 876
877
878 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 876.037 kb on - strand, within BPHYT_RS23645 at 876.170 kb on - strand at 876.260 kb on + strand at 876.324 kb on + strand at 876.325 kb on - strand at 876.327 kb on + strand at 876.412 kb on - strand at 876.519 kb on + strand, within BPHYT_RS23650 at 876.727 kb on + strand at 876.728 kb on - strand at 876.827 kb on - strand at 876.837 kb on + strand at 876.838 kb on - strand at 876.841 kb on + strand at 876.841 kb on + strand at 876.842 kb on - strand at 876.842 kb on - strand at 876.842 kb on - strand at 877.232 kb on - strand, within BPHYT_RS23655 at 877.696 kb on + strand, within BPHYT_RS23660 at 877.696 kb on + strand, within BPHYT_RS23660 at 877.697 kb on - strand, within BPHYT_RS23660 at 877.916 kb on + strand, within BPHYT_RS23660 at 877.925 kb on + strand, within BPHYT_RS23660 at 878.232 kb on - strand, within BPHYT_RS23660 at 878.270 kb on + strand, within BPHYT_RS23660 at 878.271 kb on - strand, within BPHYT_RS23660
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO6 MOI 10 remove 876,037 - BPHYT_RS23645 0.70 -1.1 876,170 - +1.1 876,260 + +0.1 876,324 + -0.9 876,325 - -0.0 876,327 + -1.5 876,412 - -0.9 876,519 + BPHYT_RS23650 0.38 -1.8 876,727 + -1.8 876,728 - +0.4 876,827 - +0.6 876,837 + +0.3 876,838 - +0.9 876,841 + +0.8 876,841 + +1.1 876,842 - -2.6 876,842 - -0.8 876,842 - -0.9 877,232 - BPHYT_RS23655 0.86 -1.2 877,696 + BPHYT_RS23660 0.20 +0.7 877,696 + BPHYT_RS23660 0.20 -3.3 877,697 - BPHYT_RS23660 0.20 -0.0 877,916 + BPHYT_RS23660 0.34 -0.5 877,925 + BPHYT_RS23660 0.34 -0.0 878,232 - BPHYT_RS23660 0.53 +0.9 878,270 + BPHYT_RS23660 0.55 -0.2 878,271 - BPHYT_RS23660 0.55 +1.0
Or see this region's nucleotide sequence