Experiment: 24hr PHYTO6 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS22840 and BPHYT_RS22845 are separated by 145 nucleotides BPHYT_RS22845 and BPHYT_RS22850 are separated by 213 nucleotides BPHYT_RS22850 and BPHYT_RS22855 are separated by 210 nucleotides BPHYT_RS22855 and BPHYT_RS22860 are separated by 126 nucleotides
BPHYT_RS22840: BPHYT_RS22840 - motility protein B, at 684,247 to 684,915
_RS22840
BPHYT_RS22845: BPHYT_RS22845 - hypothetical protein, at 685,061 to 685,309
_RS22845
BPHYT_RS22850: BPHYT_RS22850 - thioredoxin, at 685,523 to 686,089
_RS22850
BPHYT_RS22855: BPHYT_RS22855 - aldehyde-activating protein, at 686,300 to 686,671
_RS22855
BPHYT_RS22860: BPHYT_RS22860 - chemotaxis protein CheY, at 686,798 to 687,538
_RS22860
Position (kb) 685
686
687 Strain fitness (log2 ratio) -1
0
1
2
3
4 at 684.731 kb on - strand, within BPHYT_RS22840 at 684.955 kb on + strand at 684.955 kb on + strand at 685.597 kb on - strand, within BPHYT_RS22850 at 685.653 kb on + strand, within BPHYT_RS22850 at 686.152 kb on - strand at 686.152 kb on - strand at 686.557 kb on + strand, within BPHYT_RS22855 at 686.612 kb on + strand, within BPHYT_RS22855 at 686.613 kb on - strand, within BPHYT_RS22855 at 686.615 kb on - strand, within BPHYT_RS22855 at 686.817 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO6 MOI 10 remove 684,731 - BPHYT_RS22840 0.72 -0.8 684,955 + +1.2 684,955 + +0.9 685,597 - BPHYT_RS22850 0.13 -1.1 685,653 + BPHYT_RS22850 0.23 +0.3 686,152 - +0.0 686,152 - -0.0 686,557 + BPHYT_RS22855 0.69 -1.2 686,612 + BPHYT_RS22855 0.84 -0.3 686,613 - BPHYT_RS22855 0.84 -0.3 686,615 - BPHYT_RS22855 0.85 -0.5 686,817 + +4.0
Or see this region's nucleotide sequence