Experiment: 24hr PHYTO6 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS22635 and BPHYT_RS22640 are separated by 350 nucleotides BPHYT_RS22640 and BPHYT_RS22645 overlap by 4 nucleotides
BPHYT_RS22635: BPHYT_RS22635 - dihydrodipicolinate synthase, at 634,632 to 635,537
_RS22635
BPHYT_RS22640: BPHYT_RS22640 - hydroxyproline-2-epimerase, at 635,888 to 636,844
_RS22640
BPHYT_RS22645: BPHYT_RS22645 - D-amino acid oxidase, at 636,841 to 637,968
_RS22645
Position (kb) 635
636
637 Strain fitness (log2 ratio) -2
-1
0
1 at 634.909 kb on - strand, within BPHYT_RS22635 at 635.132 kb on - strand, within BPHYT_RS22635 at 635.207 kb on - strand, within BPHYT_RS22635 at 635.472 kb on + strand at 635.473 kb on - strand at 635.514 kb on + strand at 635.529 kb on - strand at 635.576 kb on - strand at 635.576 kb on - strand at 635.911 kb on + strand at 635.912 kb on - strand at 636.593 kb on + strand, within BPHYT_RS22640 at 636.719 kb on + strand, within BPHYT_RS22640 at 636.773 kb on + strand at 636.773 kb on + strand at 637.027 kb on + strand, within BPHYT_RS22645 at 637.273 kb on + strand, within BPHYT_RS22645 at 637.273 kb on + strand, within BPHYT_RS22645 at 637.416 kb on - strand, within BPHYT_RS22645 at 637.416 kb on - strand, within BPHYT_RS22645 at 637.416 kb on - strand, within BPHYT_RS22645 at 637.543 kb on + strand, within BPHYT_RS22645 at 637.705 kb on + strand, within BPHYT_RS22645 at 637.705 kb on + strand, within BPHYT_RS22645 at 637.706 kb on - strand, within BPHYT_RS22645
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO6 MOI 10 remove 634,909 - BPHYT_RS22635 0.31 -1.5 635,132 - BPHYT_RS22635 0.55 -0.9 635,207 - BPHYT_RS22635 0.63 -0.3 635,472 + -0.8 635,473 - +0.3 635,514 + +1.7 635,529 - +0.0 635,576 - +1.1 635,576 - -0.4 635,911 + +1.2 635,912 - -0.9 636,593 + BPHYT_RS22640 0.74 +0.4 636,719 + BPHYT_RS22640 0.87 +0.7 636,773 + +0.2 636,773 + +0.0 637,027 + BPHYT_RS22645 0.16 -1.6 637,273 + BPHYT_RS22645 0.38 +0.3 637,273 + BPHYT_RS22645 0.38 -1.3 637,416 - BPHYT_RS22645 0.51 +0.4 637,416 - BPHYT_RS22645 0.51 -1.4 637,416 - BPHYT_RS22645 0.51 -2.4 637,543 + BPHYT_RS22645 0.62 -0.2 637,705 + BPHYT_RS22645 0.77 -1.0 637,705 + BPHYT_RS22645 0.77 -2.6 637,706 - BPHYT_RS22645 0.77 +1.4
Or see this region's nucleotide sequence