Experiment: 24hr PHYTO6 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS21205 and BPHYT_RS21210 are separated by 116 nucleotides BPHYT_RS21210 and BPHYT_RS21215 are separated by 130 nucleotides
BPHYT_RS21205: BPHYT_RS21205 - high-affinity nickel-transporter, at 319,331 to 320,455
_RS21205
BPHYT_RS21210: BPHYT_RS21210 - hypothetical protein, at 320,572 to 321,891
_RS21210
BPHYT_RS21215: BPHYT_RS21215 - Crp/Fnr family transcriptional regulator, at 322,022 to 322,678
_RS21215
Position (kb) 320
321
322 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 319.766 kb on - strand, within BPHYT_RS21205 at 319.899 kb on + strand, within BPHYT_RS21205 at 319.900 kb on - strand, within BPHYT_RS21205 at 320.050 kb on - strand, within BPHYT_RS21205 at 320.211 kb on + strand, within BPHYT_RS21205 at 320.211 kb on + strand, within BPHYT_RS21205 at 320.451 kb on + strand at 320.606 kb on + strand at 320.618 kb on + strand at 320.618 kb on + strand at 320.696 kb on + strand at 320.696 kb on + strand at 320.697 kb on - strand at 320.697 kb on - strand at 320.735 kb on + strand, within BPHYT_RS21210 at 320.996 kb on + strand, within BPHYT_RS21210 at 321.208 kb on - strand, within BPHYT_RS21210 at 321.407 kb on - strand, within BPHYT_RS21210 at 321.422 kb on + strand, within BPHYT_RS21210 at 321.422 kb on + strand, within BPHYT_RS21210 at 321.423 kb on - strand, within BPHYT_RS21210 at 321.749 kb on + strand, within BPHYT_RS21210 at 322.023 kb on + strand at 322.024 kb on - strand at 322.324 kb on + strand, within BPHYT_RS21215 at 322.324 kb on + strand, within BPHYT_RS21215 at 322.324 kb on + strand, within BPHYT_RS21215 at 322.324 kb on + strand, within BPHYT_RS21215 at 322.325 kb on - strand, within BPHYT_RS21215 at 322.325 kb on - strand, within BPHYT_RS21215 at 322.545 kb on + strand, within BPHYT_RS21215 at 322.546 kb on - strand, within BPHYT_RS21215 at 322.546 kb on - strand, within BPHYT_RS21215 at 322.575 kb on + strand, within BPHYT_RS21215 at 322.813 kb on + strand at 322.813 kb on + strand at 322.813 kb on + strand at 322.845 kb on + strand at 322.846 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO6 MOI 10 remove 319,766 - BPHYT_RS21205 0.39 +1.4 319,899 + BPHYT_RS21205 0.50 +0.4 319,900 - BPHYT_RS21205 0.51 +0.3 320,050 - BPHYT_RS21205 0.64 +1.7 320,211 + BPHYT_RS21205 0.78 -2.1 320,211 + BPHYT_RS21205 0.78 -0.9 320,451 + -0.4 320,606 + -2.6 320,618 + +0.9 320,618 + -1.5 320,696 + -0.2 320,696 + -0.2 320,697 - -1.5 320,697 - -0.1 320,735 + BPHYT_RS21210 0.12 -0.9 320,996 + BPHYT_RS21210 0.32 -2.9 321,208 - BPHYT_RS21210 0.48 -0.4 321,407 - BPHYT_RS21210 0.63 +2.4 321,422 + BPHYT_RS21210 0.64 -0.5 321,422 + BPHYT_RS21210 0.64 -1.3 321,423 - BPHYT_RS21210 0.64 -0.9 321,749 + BPHYT_RS21210 0.89 -0.2 322,023 + +1.0 322,024 - -1.6 322,324 + BPHYT_RS21215 0.46 +0.0 322,324 + BPHYT_RS21215 0.46 +0.8 322,324 + BPHYT_RS21215 0.46 +0.0 322,324 + BPHYT_RS21215 0.46 -0.6 322,325 - BPHYT_RS21215 0.46 -1.2 322,325 - BPHYT_RS21215 0.46 +0.1 322,545 + BPHYT_RS21215 0.80 -0.2 322,546 - BPHYT_RS21215 0.80 +0.8 322,546 - BPHYT_RS21215 0.80 -1.6 322,575 + BPHYT_RS21215 0.84 +1.1 322,813 + +1.4 322,813 + +0.0 322,813 + +0.1 322,845 + -1.4 322,846 - -0.6
Or see this region's nucleotide sequence