Experiment: 24hr PHYTO6 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS21045 and BPHYT_RS21050 are separated by 523 nucleotides BPHYT_RS21050 and BPHYT_RS21055 are separated by 2 nucleotides
BPHYT_RS21045: BPHYT_RS21045 - bis(5'-nucleosyl)-tetraphosphatase, at 281,719 to 282,801
_RS21045
BPHYT_RS21050: BPHYT_RS21050 - aldehyde-activating protein, at 283,325 to 283,900
_RS21050
BPHYT_RS21055: BPHYT_RS21055 - S-formylglutathione hydrolase, at 283,903 to 284,733
_RS21055
Position (kb) 283
284 Strain fitness (log2 ratio) -2
-1
0
1
2 at 282.618 kb on - strand, within BPHYT_RS21045 at 282.638 kb on + strand, within BPHYT_RS21045 at 282.683 kb on - strand, within BPHYT_RS21045 at 282.818 kb on - strand at 282.921 kb on + strand at 282.970 kb on + strand at 282.979 kb on + strand at 282.979 kb on + strand at 282.979 kb on + strand at 283.080 kb on + strand at 283.297 kb on + strand at 283.301 kb on - strand at 283.304 kb on - strand at 283.398 kb on + strand, within BPHYT_RS21050 at 283.398 kb on + strand, within BPHYT_RS21050 at 283.474 kb on + strand, within BPHYT_RS21050 at 283.632 kb on - strand, within BPHYT_RS21050 at 283.831 kb on - strand, within BPHYT_RS21050 at 284.007 kb on + strand, within BPHYT_RS21055 at 284.131 kb on - strand, within BPHYT_RS21055 at 284.264 kb on - strand, within BPHYT_RS21055 at 284.497 kb on + strand, within BPHYT_RS21055 at 284.668 kb on - strand at 284.668 kb on - strand at 284.675 kb on - strand at 284.810 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO6 MOI 10 remove 282,618 - BPHYT_RS21045 0.83 -0.4 282,638 + BPHYT_RS21045 0.85 +0.0 282,683 - BPHYT_RS21045 0.89 -0.5 282,818 - +0.4 282,921 + -0.2 282,970 + -0.4 282,979 + -1.6 282,979 + -1.1 282,979 + +0.0 283,080 + +0.5 283,297 + +0.4 283,301 - +1.4 283,304 - -0.7 283,398 + BPHYT_RS21050 0.13 -0.3 283,398 + BPHYT_RS21050 0.13 -1.9 283,474 + BPHYT_RS21050 0.26 -0.9 283,632 - BPHYT_RS21050 0.53 +0.0 283,831 - BPHYT_RS21050 0.88 +1.0 284,007 + BPHYT_RS21055 0.13 -0.6 284,131 - BPHYT_RS21055 0.27 +1.9 284,264 - BPHYT_RS21055 0.43 -1.1 284,497 + BPHYT_RS21055 0.71 +0.3 284,668 - -0.4 284,668 - -0.4 284,675 - -1.8 284,810 - +1.2
Or see this region's nucleotide sequence